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FGFR3 Antibody (136312) [Unconjugated] - (IIIc)

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 (1 Review)
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HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FGFR3 (IIIc) Monoclonal Antibody (Catalog # MAB7662, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FGFR3 (IIIc) Monoclonal Antibody (Catalog # MAB7662, filled histogram) or isotype control antibody (MAB002, open histogram), followed by ...read more
FGFR3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human FGFR3 (IIIc) Monoclonal ...read more
FGFR3 was detected in immersion fixed MCF‑7 human breast cancer cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human FGFR3 (IIIc) Monoclonal ...read more
FGF R3 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human FGF R3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 8 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, ICC/IF
Clone
136312
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7662-SP
Carrier Free
MAB7662-500
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FGFR3 Antibody (136312) [Unconjugated] - (IIIc) Summary

Additional Information
Clone 136312 was used by HLDA to establish CD designation
Immunogen
Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)
Specificity
Detects human FGF R3 (IIIc) in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) FGF R2 (IIIc), recombinant mouse (rm) FGF R2 (IIIc) and rmFGF R3 (IIIc) is observed. In Western blots (non-reducing conditions only), 50‑100% cross-reactivity with rhFGF R2 (IIIc), rmFGF R2 (IIIc) and rmFGF R3 (IIIc) is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
MAB7662 in the following applications:
Publications
Read Publications using
MAB7662 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR3 Antibody (136312) [Unconjugated] - (IIIc)

  • CD333
  • CEK
  • EC 2.7.10
  • FGF R3
  • FGFR3
  • fibroblast growth factor receptor 3
  • HSFGFR3EX
  • JTK4
  • JTK4thanatophoric dwarfism

Background

FGF R3 is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co-factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF-1, -2, -4, -8b, -8e, -8f, -9 and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB7662
FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
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Publications for FGFR3 Antibody (MAB7662)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA Capture, Immunoprecipitation.


Filter By Application
ELISA Capture
(1)
Immunoprecipitation
(1)
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Human
(1)
Mouse
(1)
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Review for FGFR3 Antibody (MAB7662) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB7662:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry FGFR3 MAB7662
Enlarge 		3
reviewed by:
Natalie Erdmann
 ICC Human 01/26/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedKMS11, LP-1 and RPMI8226 multiple myeloma cell lines
SpeciesHuman
LotGHK051512A

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Recent Reviews

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1
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Immunocytochemistry FGFR3 MAB7662
Enlarge
 Natalie Erdmann
01/26/2016
Application: ICC
Species: Human
read this review

Bioinformatics

Gene Symbol FGFR3