![Western Blot: FEN-1 Antibody [NB100-321] - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Western-Blot-NB100-321-img0012.jpg "Western Blot: FEN-1 Antibody [NB100-321] - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Bv, ShSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237). |
| Predicted Species | Bovine (100%), Sheep (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FEN1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||||
| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. 16-18 hour incubation is recommended |
||||
| Control |
|
||||
| Publications |
|
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for FEN-1 Antibody (NB100-321)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FEN1 |
| Entrez |
|
| Uniprot |
|
![Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321] - FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Fen1 (NB100-321) used at a dilution of 1:1000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunohistochemistry-NB100-321-img0013.jpg "Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321] - FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Fen1 (NB100-321) used at a dilution of 1:1000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: FEN-1 Antibody [NB100-321] - Detection of human Fen1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 (lot NB100-321-2) used for IP at 6 ug per reaction. Fen1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-321-1). For blotting immunoprecipitated Fen1, NB100-321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunoprecipitation-NB100-321-img0014.jpg "Immunoprecipitation: FEN-1 Antibody [NB100-321] - Detection of human Fen1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 (lot NB100-321-2) used for IP at 6 ug per reaction. Fen1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-321-1). For blotting immunoprecipitated Fen1, NB100-321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")