![Western Blot: Elk-1 [p Ser383] Antibody [NBP3-05380] - Western blot analysis of extracts of Jurkat cells, using Elk-1 antibody (NBP3-05380) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Elk-1-[p-Ser383]-Antibody-Western-Blot-NBP3-05380-img0002.jpg "Western Blot: Elk-1 [p Ser383] Antibody [NBP3-05380] - Western blot analysis of extracts of Jurkat cells, using Elk-1 antibody (NBP3-05380) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | A synthetic phosphorylated peptide around S383 of human ELK1 (NP_005220.2). TLSPI |
| Modification | p Ser383 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ELK1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Affinity purified |
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
![Elk-1 [p Ser383] Antibody - BSA Free](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Elk-1 [p Ser383] Antibody - BSA Free")
Secondary Antibodies |
Isotype Controls |
Research Areas for Elk-1 Antibody (NBP3-05380)Find related products by research area.
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| Gene Symbol | ELK1 |
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-52202516283228.jpg "Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-5220251640288.jpg "Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Mouse cerebellum tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-52202516345237.jpg "Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Mouse cerebellum tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-5220251636368.jpg "Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")