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Elk-1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Staining in human ovary and pancreas tissues using anti-ELK1 antibody. Corresponding ELK1 RNA-seq data are presented for the ...read more
Immunocytochemistry/ Immunofluorescence: Elk-1 Antibody [NBP2-38278] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Elk-1 Antibody [NBP2-38278] - Staining of human ovary shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Elk-1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HPRPAVVLPSAAPAGAAAPPSGSRSTSPSPLEACLEAEEAGLPLQVILTPPEAPNLKSEELNVEPGLGRALPPEVKVEGPKEE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ELK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Elk-1 Protein (NBP2-38278PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Elk-1 Antibody

  • Elk1
  • Elk-1
  • ELK1, member of ETS oncogene family
  • ETS domain-containing protein Elk-1
  • ETS-like gene 1

Background

ELK is a member of the family of genes homologous to the v-Ets oncogene isolated from the E26 eythroblastosis virus. Members of this family exhibit varied patterns of tissue expression. They also share a highly conserved C-terminal domain that may be responsible for the DNA binding activity of all members of the Ets gene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Elk-1 Products

Array NBP2-38278

Research Areas for Elk-1 Antibody (NBP2-38278)

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Blogs on Elk-1

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Bioinformatics

Gene Symbol ELK1
Uniprot