![Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB110-59723-img0002.jpg "Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.")
| Reactivity | Hu, Mu, PoSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide made to a portion of human EGR2 (within residues 200-300). [Swiss-Prot# P11161] |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EGR2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This EGR2 antibody is useful for ICC and Western blot, where a band is seen at ~50 kDa. There is also a strong non-specific band at ~75 kDa. In ICC/IF nuclear staining was observed in Hela cells. |
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| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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Enlarge |
reviewed by:
Daochun Sun |
IHC-P | Mouse | 02/24/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for EGR2 Antibody (NB110-59723)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EGR2 |
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-EGR2 Antibody NB110-59723 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-59723-img0007.jpg "Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-EGR2 Antibody NB110-59723 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: EGR2 Antibody [NB110-59723] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of EGR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunohistochemistry-Paraffin-NB110-59723-img0008.jpg "Immunohistochemistry-Paraffin: EGR2 Antibody [NB110-59723] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of EGR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 staining on E13.5 dorsal root ganglion and neural tube.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunofluorescence-NB110-59723-img0006.jpg "Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 staining on E13.5 dorsal root ganglion and neural tube.")
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