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![Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB110-59723-img0002.jpg "Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.")
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-EGR2 Antibody NB110-59723 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-59723-img0007.jpg)
![Immunohistochemistry-Paraffin: EGR2 Antibody [NB110-59723] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of EGR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunohistochemistry-Paraffin-NB110-59723-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 staining on E13.5 dorsal root ganglion and neural tube.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunofluorescence-NB110-59723-img0006.jpg)
![Western Blot: EGR2 Antibody [NB100-92327] - Analysis of pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB100-92327-img0004.jpg "Western Blot: EGR2 Antibody [NB100-92327] - Analysis of pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)")
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB100-92327] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EGR2 at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-92327-img0005.jpg)
![Immunohistochemistry-Paraffin: EGR2 Antibody [NB100-92327] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse brain using EGR2 antibody at 1:200 dilution. The signal was detected using HRP-conjugated anti-rabbit secondary antibody and DAB based detection reagent, and the sections were further counterstained with hematoxylin. This EGR2 antibody generated primarily a nuclear staining signal in the neurons and the glial cells, however, some cytoplasmic staining was also observed. The cytoplasmic signal potentially represents the translational or 14-3-3sigma interacting cytoplasmic pool of EGR2.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunohistochemistry-Paraffin-NB100-92327-img0006.jpg)
![Western Blot: EGR2 Antibody [NB100-92327] - Egr-2 Antibody in extracts from HUVEC cells.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB100-92327-img0001.jpg)
![Western Blot: EGR2 Antibody [NBP2-16286] - Expression in transfected 293T cell line. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NBP2-16286-img0001.jpg "Western Blot: EGR2 Antibody [NBP2-16286] - Expression in transfected 293T cell line. A: Non-transfected lysate. B: EGR2 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Frozen: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of mouse brain tissue (paraformaldehyde-fixed frozen sections), using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/EGR2-Antibody-Immunohistochemistry-Frozen-NBP2-16286-img0002.jpg)
![Western Blot: EGR2 Antibody [NBP2-16286] - Sample (30 ug of whole cell lysate) A: zebrafish brain 10% SDS PAGE NBP2-16286 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16286_rabbit-polyclonal-egr2-antibody-91020242063357.jpg)
![Immunohistochemistry: EGR2 Antibody [NBP2-16286] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using EGR2 antibody (NBP2-16286) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16286_rabbit-polyclonal-egr2-antibody-91020242014619.jpg)
![Western Blot: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGR2.](http://images.novusbio.com/fullsize/EGR2-Antibody-OTI1B12-Western-Blot-NBP2-45696-img0004.jpg "Western Blot: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGR2.")
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/EGR2-Antibody-OTI1B12-Immunofluorescence-NBP2-45696-img0002.jpg)
![Immunohistochemistry: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/EGR2-Antibody-OTI1B12-Immunohistochemistry-NBP2-45696-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY EGR2.](http://images.novusbio.com/fullsize/EGR2-Antibody-OTI1B12-Immunofluorescence-NBP2-45696-img0001.jpg)
![Western Blot: EGR2 Antibody (1F6V7) [NBP3-16157] - Western blot analysis of extracts of various cell lines, using EGR2 Rabbit mAb (NBP3-16157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/EGR2-Antibody-1F6V7-Western-Blot-NBP3-16157-img0001.jpg "Western Blot: EGR2 Antibody (1F6V7) [NBP3-16157] - Western blot analysis of extracts of various cell lines, using EGR2 Rabbit mAb (NBP3-16157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunoprecipitation: EGR2 Antibody (1F6V7) [NBP3-16157] - Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug EGR2 antibody (NBP3-16157). Western blot was performed from the immunoprecipitate using EGR2 antibody (NBP3-16157) at a dilition of 1:500.](http://images.novusbio.com/fullsize/EGR2-Antibody-1F6V7-Immunoprecipitation-NBP3-16157-img0002.jpg)
![Western Blot: EGR2 Antibody (27C3) [NBP3-26605] - Positive Western Blot detected in: SY5Y whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate. All lanes: EGR2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp3-26605_rabbit-egr2-mab-27c3-2822024115250.jpg "Western Blot: EGR2 Antibody (27C3) [NBP3-26605] - Positive Western Blot detected in: SY5Y whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate. All lanes: EGR2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50 kDa Observed band size: 50 kDa")
![Flow Cytometry: EGR2 Antibody (27C3) [NBP3-26605] - Overlay Peak curve showing SH-SY5Y cells stained with EGR2 Antibody (27C3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26605_rabbit-egr2-mab-27c3-282202414312741.jpg)
![Western Blot: EGR2 Antibody [NB100-68146] - Staining of lysates of cell line HepG2 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/EGR2-Antibody-Western-Blot-NB100-68146-img0005.jpg "Western Blot: EGR2 Antibody [NB100-68146] - Staining of lysates of cell line HepG2 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![SDS-Page: Recombinant Human EGR2 Protein [H00001959-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001959-Q01-1.jpg "SDS-Page: Recombinant Human EGR2 Protein [H00001959-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Human EGR2 ELISA Kit (Colorimetric) [NBP3-08108] - Samples were spiked with high concentrations of Human EGR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08108_human-egr2-elisa-kit-colorimetric-132202416133622.jpg "ELISA: Human EGR2 ELISA Kit (Colorimetric) [NBP3-08108] - Samples were spiked with high concentrations of Human EGR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human EGR2 ELISA Kit (Colorimetric) [NBP3-08108] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08108_human-egr2-elisa-kit-colorimetric-432024924051.jpg)
![ELISA: Mouse EGR2 ELISA Kit (Colorimetric) [NBP3-08109] - Samples were spiked with high concentrations of Mouse EGR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08109_mouse-egr2-elisa-kit-colorimetric-13220241692232.jpg "ELISA: Mouse EGR2 ELISA Kit (Colorimetric) [NBP3-08109] - Samples were spiked with high concentrations of Mouse EGR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse EGR2 ELISA Kit (Colorimetric) [NBP3-08109] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08109_mouse-egr2-elisa-kit-colorimetric-432024973699.jpg)