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EGR4 Antibody

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Western Blot: EGR4 Antibody [NBP1-06032] - (0.3ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: EGR4 Antibody [NBP1-06032] - (0.3ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: EGR4 Antibody [NBP1-06032] - NBP1-06032 (0.3ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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EGR4 Antibody Summary

Immunogen
Peptide with sequence NCGSQGDYQAAPE corresponding to internal region according to NP_001956.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EGR4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 50.8 kDa band according to NP_001956.2).
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-06032.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EGR4 Antibody

  • AT133
  • early growth response 4
  • early growth response protein 4
  • EGR-4
  • NGFIC
  • NGFI-C
  • PAT133

Background

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates thetranscription of target genes whose products are required for mitogenesis and differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGR4 Antibody (NBP1-06032)(1)


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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EGR4
Entrez
Uniprot