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Nab2 Antibody

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Western Blot: Nab2 Antibody [NBP2-19462] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:2000.
Western Blot: Nab2 Antibody [NBP2-19462] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: Nab2 Antibody [NBP2-19462] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Nab2 Antibody [NBP2-19462] - Mouse fore brain. NAB2 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Immunoprecipitation: Nab2 Antibody [NBP2-19462] - Protein from Jurkat whole cell extracts using 5 ug of NAB2 antibody. Western blot analysis was performed using NAB2 antibody. EasyBlot anti-Rabbit IgG was used as a ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Nab2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Nab2. The exact sequence is proprietary.
Localization
Nucleus (By similarity)
Predicted Species
Rat (98%), Bovine (99%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAB2
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Nab2 Antibody

  • EGR-1-binding protein 2
  • MADEREGR1 binding protein 2
  • Melanoma-associated delayed early response protein
  • MGC75085
  • NGFI-A binding protein 2 (EGR1 binding protein 2)
  • NGFI-A-binding protein 2
  • Protein MADER

Background

This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-19462
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Applications: WB, ICC/IF, IHC, IP
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NAB2
Uniprot