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MafB Antibody (372239) [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK were included. A specific band for MafB was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog ...read more
MafB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Rat MafB Monoclonal Antibody (Catalog # MAB3810) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
Simple Western lane view shows lysates of MDA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for MafB at approximately 53 kDa (as indicated) using 5 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
372239
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3810-SP
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MafB Antibody (372239) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MafB
Met1-Leu323
Accession # Q9Y5Q3
Specificity
Detects human and rat MafB at in Western blots. No cross-reactivity with recombinant human (rh) MafF, rhMafG, or rhMafK is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAFB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB3810 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MafB Antibody (372239) [Unconjugated]

  • Kreisler (mouse) maf-related leucine zipper homolog
  • Kreisler maf-related leucine zipper homolog
  • KRML
  • KRMLMAFB/Kreisler basic region/leucine zipper transcription factor
  • MafB
  • Maf-B
  • MGC43127
  • transcription factor MafB
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog B

Background

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. MafB has been implicated in the regulation of hindbrain development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MafB Antibody (MAB3810)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


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Product General Protocols

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Bioinformatics

Gene Symbol MAFB
Uniprot