EGF Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha , amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1217 amino acid (aa) mouse EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature mouse EGF shares 70% and 77% aa sequence identity with mature human and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, Neutralization.

Showing Publications 1 - 8 of 8.

Publications using AF2028	Applications	Species
JO Lamontagne, H Zhang, AM Zeid, K Strittmatt, AD Rocha, T Williams, S Zhang, AG Marneros Transcription factors AP-2alpha and AP-2beta regulate distinct segments of the distal nephron in the mammalian kidney Nature Communications, 2022-04-25;13(1):2226. 2022-04-25 [PMID: 35468900] (IHC, Mouse)	IHC	Mouse
AG Marneros Magnesium and Calcium Homeostasis Depend on KCTD1 Function in the Distal Nephron Cell Reports, 2021-01-12;34(2):108616. 2021-01-12 [PMID: 33440155] (IHC, Mouse, Transgenic Mouse)	IHC	Mouse, Transgenic Mouse
J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth Redox Biol, 2019-02-06;22(0):101116. 2/6/2019 [PMID: 30769285]
A critical role for NF2 and the Hippo pathway in branching morphogenesis Nat Commun, 2016-08-02;7(0):12309. 2016-08-02 [PMID: 27480037] (Neutralization, Mouse)	Neutralization	Mouse
Ohkubo , Hirotoki, Ito , Yoshiya, Minamino , Tsutomu, Eshima , Koji, Kojo , Ken, Okizaki , Shin-ich, Hirata , Mitsuhir, Shibuya , Masabumi, Watanabe , Masahiko, Majima , Masataka VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury. PLoS ONE, 2014-08-27;9(8):e105533. 2014-08-27 [PMID: 25162491] (IHC, Mouse)	IHC	Mouse
Ohkubo , Hirotoki, Ito , Yoshiya, Minamino , Tsutomu, Mishima , Toshiaki, Hirata , Mitsuhir, Hosono , Kanako, Shibuya , Masabumi, Yokomizo , Takehiko, Shimizu , Takao, Watanabe , Masahiko, Majima , Masataka Leukotriene B4 type-1 receptor signaling promotes liver repair after hepatic ischemia/reperfusion injury through the enhancement of macrophage recruitment. FASEB J, 2013-04-29;27(8):3132-43. 2013-04-29 [PMID: 23629862] (IHC-Fr, Neutralization, Mouse)	IHC-Fr, Neutralization	Mouse
Lorena Hernandez, Marco A O Magalhaes, Salvatore J Coniglio, John S Condeelis, Jeffrey E Segall Opposing roles of CXCR4 and CXCR7 in breast cancer metastasis Breast Cancer Research 12/9/2011 [PMID: 22152016]
Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages. J. Clin. Invest., 2011-04-25;121(5):1692-708. 2011-04-25 [PMID: 21519141] (Neutralization, Mouse)	Neutralization	Mouse

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-Fr	Mouse	01/06/2015	View Summary Application Immunohistochemistry-Frozen Sample Tested See PMID 23074208 Species Mouse

Array AF2028

• EGF Antibodies
• EGF ELISA Kit
• EGF Lysate
• EGF Proteins
• EGF ELISAs
• EGF Proteins and Enzymes