We have used one of your antibodies against Dio3 (cat# NBP1-05767) in the past and, despite it being directed against rodent D3 peptide sequence, it also appears to stains human D3 specifically in our hands. Can you please let us know the rodent D3 peptide sequence that was used to create this antibody? Alternatively, can you let us know if this rodent D3 peptide sequence also matches the human D3 peptide sequence? If there is a high degree of sequence homology, we wish to publish our findings and include a description that your antibody works in human tissues (IHC).We also noticed a new D3 aB on your website (cat# NBP1-19747) that is said to recognize human D3. Can you also let me know the sequence of the peptide used to generate it? We are one of the only labs in the world that study D3. If you can send us some samples of these antibodies, we can likely help you validate them experimentally for Western, IHC, etc.

Name: DIO3 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-05767
Rating: 3.5 (2 reviews)

Question

Accepted Answer

The immunogen shares 93% homology to human. It is expected to cross-react with human cells. Furthermore, full-length DIO3 in human and mouse share greater than 95% similarity over the entire protein. It will be very difficult to find an antibody that works on one species but not the other unless the immunogen falls within amino acids 48-55, which is the only region where there is significant difference between the two species. It is highly doubtful you will find a commercial antibody that uses this immunogen to generate their antibody as most companies will want to increase the amount of species recognized by an antibody, not decrease it. For NBP1-19747, like all of our immunogens, the exact sequence is considered proprietary, but as you can see on our datasheet, the immunogen surrounds amino acid 66, which does not fall within the range where mouse and human are dissimilar.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Ha, RbSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP1-05767AF350

Alexa Fluor 405 NBP1-05767AF405

Alexa Fluor 488 NBP1-05767AF488

Alexa Fluor 532 NBP1-05767AF532

Alexa Fluor 594 NBP1-05767AF594

Alexa Fluor 647 NBP1-05767AF647

Alexa Fluor 700 NBP1-05767AF700

Alexa Fluor 750 NBP1-05767AF750

Allophycocyanin NBP1-05767APC

Biotin NBP1-05767B

DyLight 350 NBP1-05767UV

DyLight 405 NBP1-05767V

DyLight 488 NBP1-05767G

DyLight 550 NBP1-05767R

DyLight 594 NBP1-05767DL594

DyLight 650 NBP1-05767C

DyLight 680 NBP1-05767FR

DyLight 755 NBP1-05767IR

FITC NBP1-05767F

HRP NBP1-05767H

Janelia Fluor 549 NBP1-05767JF549

Janelia Fluor 646 NBP1-05767JF646

PE NBP1-05767PE

PerCP NBP1-05767PCP

mFluor Violet 450 SE NBP1-05767MFV450

mFluor Violet 500 SE NBP1-05767MFV500

mFluor Violet 610 SE NBP1-05767MFV610

Catalog# & Formulation	Size	Price
BSA Free NBP1-05767SS

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-05767AF350
Alexa Fluor 405	NBP1-05767AF405
Alexa Fluor 488	NBP1-05767AF488
Alexa Fluor 532	NBP1-05767AF532
Alexa Fluor 594	NBP1-05767AF594
Alexa Fluor 647	NBP1-05767AF647
Alexa Fluor 700	NBP1-05767AF700
Alexa Fluor 750	NBP1-05767AF750
Allophycocyanin	NBP1-05767APC
Biotin	NBP1-05767B
DyLight 350	NBP1-05767UV
DyLight 405	NBP1-05767V
DyLight 488	NBP1-05767G
DyLight 550	NBP1-05767R
DyLight 594	NBP1-05767DL594
DyLight 650	NBP1-05767C
DyLight 680	NBP1-05767FR
DyLight 755	NBP1-05767IR
FITC	NBP1-05767F
HRP	NBP1-05767H
Janelia Fluor 549	NBP1-05767JF549
Janelia Fluor 646	NBP1-05767JF646
PE	NBP1-05767PE
PerCP	NBP1-05767PCP
mFluor Violet 450 SE	NBP1-05767MFV450
mFluor Violet 500 SE	NBP1-05767MFV500
mFluor Violet 610 SE	NBP1-05767MFV610

DIO3 Antibody - BSA Free

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Publications for DIO3 Antibody (NBP1-05767)(18)

Reviews for DIO3 Antibody (NBP1-05767) (2) 3.52

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Immunogen	Synthetic peptide made to an internal portion of rat DIO3 (within residues 250-300). [Swiss-Prot# P49897]
Localization	Cell membrane; Single-pass type II membrane protein
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	DIO3
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS and 30% Glycerol
Preservative	0.1% Sodium Azide
Concentration	1 mg/ml
Purity	Immunogen affinity purified

Publications using NBP1-05767	Applications	Species
Lavecchia AM, Mantzouratou P, Cerullo D et al. Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs iScience 2023-10-20 [PMID: 37752946] (IHC)	IHC
Peluso T, Nittoli V, Reale C et al. Chronic Exposure to Chlorpyrifos Damages Thyroid Activity and Imbalances Hepatic Thyroid Hormones Signaling and Glucose Metabolism: Dependency of T3-FOXO1 Axis by Hyperglycemia International journal of molecular sciences 2023-05-31 [PMID: 37298533] (WB, Mouse) Details: Dilution: 1:3000	WB	Mouse
Kohon MY, Zaaroor Levy M, Hornik-Lurie T et al. ?v?3 Integrin as a Link between the Development of Fibrosis and Thyroid Hormones in Systemic Sclerosis International journal of molecular sciences 2023-05-18 [PMID: 37240272] (WB, Human)	WB	Human
Salas-Lucia F, Fekete C, Sinkó R et al. Axonal T3 uptake and transport can trigger thyroid hormone signaling in the brain eLife 2023-05-19 [PMID: 37204837] (ICC/IF, IHC, Mouse, Rat)	ICC/IF, IHC	Mouse, Rat
Agarwal S, Koh KH, Tardi NJ Et al. Deiodinase-3 is a thyrostat to regulate podocyte homeostasis EBioMedicine 2021-10-01 [PMID: 34649077] (WB, ICC/IF, PLA, Mouse, Human)	WB, ICC/IF, PLA	Mouse, Human
Devalraju Kp, Tripathi D, Neela Vsk Et Al. Reduced thyroxine production in young household contacts of tuberculosis patients increases active tuberculosis disease risk JCI insight 2021-07-08 [PMID: 34236051] (WB)	WB
Benedetti V, Lavecchia AM, Locatelli M et al. Alteration of thyroid hormone signaling triggers the diabetes-induced pathological growth, remodeling, and dedifferentiation of podocytes JCI Insight 2019-09-19 [PMID: 31534055] (IHC-P, Rat)	IHC-P	Rat
Porings AS, Lowin T, Dufner B et al. A thyroid hormone network exists in synovial fibroblasts of rheumatoid arthritis and osteoarthritis patients Sci Rep [PMID: 31519956] (IHC-Fr, Human)	IHC-Fr	Human
Calonne J, Isacco L, Miles-Chan J et al. Reduced Skeletal Muscle Protein Turnover and Thyroid Hormone Metabolism in Adaptive Thermogenesis That Facilitates Body Fat Recovery During Weight Regain Front Endocrinol (Lausanne) 2019-02-28 [PMID: 30873123] (WB, Rat)	WB	Rat
Yamauchi I, Sakane Y, Yamashita T et al. Effects of growth hormone on thyroid function are mediated by type 2 iodothyronine deiodinase in humans. Endocrine 2017-12-22 [PMID: 29274063] (WB, Human)	WB	Human
De Andrade PB, Neff LA, Strosova MK et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol 2015-01-01 [PMID: 26441673]
Castroneves LA, Jugo RH, Maynard MA et al. Mice with hepatocyte-specific deficiency of type 3 deiodinase have intact liver regeneration and accelerated recovery from nonthyroidal illness after toxin-induced hepatonecrosis. Endocrinology 2014-07-08 [PMID: 25004090] (IHC-P, Mouse) Details: DIO3 antibody used at 1:2000 dilution for IHC-P alongwith isotype negative controls on wild-type D3 /D3flox allele and LD3KO/recombined D3null allele mice treated with CCl4. DIO3 positivity was seen in hepatocytes of D3-flox but not in LD3KO mice (Figure 2D).	IHC-P	Mouse
Medina MC, Foseca T, Molina J et al. Maternal Inheritance of an Inactive Type III Deiodinase Gene Allele Affects Mouse Pancreatic b-cells and Disrupts Glucose Homeostasis Endocrinology. 2014-06-02 [PMID: 24885572] (IHC-Fr, ICC/IF, Mouse) Details: D3 control peptide used as a negative control (used for blocking NBP1-05767 ) and no staining observed in antibody preincubated with the peptide in IHC-Fr assay on mouse tissues.	IHC-Fr, ICC/IF	Mouse
Maynard MA, Marino-Enriquez A, Fletcher JA et al. Thyroid hormone inactivation in gastrointestinal stromal tumors. N Engl J Med 2014-04-03 [PMID: 24693892] (IHC-P, Human) Details: Using the Biotin conjugated version of NBP1-05767, catalog number NBP1-05767B.	IHC-P	Human
Vose LR, Vinukonda G, Jo S et al. Treatment with thyroxine restores myelination and clinical recovery after intraventricular hemorrhage. J Neurosci. 2013-10-30 [PMID: 24174657] (ICC/IF, IF/IHC, Human)	ICC/IF, IF/IHC	Human
Jo S, Kallo I, Bardoczi Z, Arrojo E et al. Neuronal Hypoxia Induces Hsp40-Mediated Nuclear Import of Type 3 Deiodinase As an Adaptive Mechanism to Reduce Cellular Metabolism J Neurosci 2012-06-20 [PMID: 22723689] (WB, Hamster, Human)	WB	Hamster, Human
Kallo I, Mohacsik P, Vida B et al. A Novel Pathway Regulates Thyroid Hormone Availability in Rat and Human Hypothalamic Neurosecretory Neurons PLoS One 2012-01-01 [PMID: 22719854] (WB, Human, Rat)	WB	Human, Rat
Freitas BC, Gereben B, Castillo M et al. Paracrine signaling by glial cell-derived triiodothyronine activates neuronal gene expression in the rodent brain and human cells. J Clin Invest 2010-06-01 [PMID: 20458138] Details: Using the Biotin conjugated version of NBP1-05767, catalog number NBP1-05767B.
Show All 18 Publications. Collapse Publications.

	Linnea Vose 01/28/2013
Application:	WB
Species:	Other

	Verified Customer 01/05/2010
Application:	WB
Species:	Human