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DIO3 Antibody [Alexa Fluor® 647]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 647

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-05767AF350
Alexa Fluor 405
NBP1-05767AF405
Alexa Fluor 488
NBP1-05767AF488
Alexa Fluor 532
NBP1-05767AF532
Alexa Fluor 594
NBP1-05767AF594
Alexa Fluor 700
NBP1-05767AF700
Alexa Fluor 750
NBP1-05767AF750
Allophycocyanin
NBP1-05767APC
Biotin
NBP1-05767B
DyLight 350
NBP1-05767UV
DyLight 405
NBP1-05767V
DyLight 488
NBP1-05767G
DyLight 550
NBP1-05767R
DyLight 594
NBP1-05767DL594
DyLight 650
NBP1-05767C
DyLight 680
NBP1-05767FR
DyLight 755
NBP1-05767IR
FITC
NBP1-05767F
HRP
NBP1-05767H
Janelia Fluor 549
NBP1-05767JF549
Janelia Fluor 646
NBP1-05767JF646
PE
NBP1-05767PE
PerCP
NBP1-05767PCP
Unconjugated
NBP1-05767
mFluor Violet 450 SE
NBP1-05767MFV450
mFluor Violet 500 SE
NBP1-05767MFV500
mFluor Violet 610 SE
NBP1-05767MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-05767
BSA Free
NBP1-05767SS
BSA Free
NBP1-05767
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DIO3 Antibody [Alexa Fluor® 647] Summary

Immunogen
Synthetic peptide made to an internal portion of rat DIO3 (within residues 250-300). [Swiss-Prot# P49897]
Localization
Cell membrane; Single-pass type II membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIO3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rabbit reactivity via customer review.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DIO3 Antibody [Alexa Fluor® 647]

  • 5DIII
  • D3
  • deiodinase, iodothyronine, type III
  • DIOIII
  • EC 1.97.1
  • EC 1.97.1.11
  • ITDI3
  • placental type
  • thyroxine deiodinase type III (selenoprotein)
  • Type 3 DI
  • type 3 iodothyronine selenodeiodinase
  • type III iodothyronine deiodinase
  • type-III 5' deiodinase
  • Type-III 5'-deiodinase

Background

Iodothyronine deiodinase 3 (DIO3) is a peroxidase enzyme that is involved in the activation or deactivation of thyroid hormones. Specifically, DIO-3 deiodinizes the thyroid hormones T4 and T3 into their respective inactive metabolites, RT3 and T2.

Dio3 has an essential role for regulation of thyroid hormone inactivation during embryological development, and may play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Dio3 has been linked to the development of consumptive hypothyroidism in both infants and adults.

DIO3 antibodies are useful tools for hormone regulation research and in some embryological development studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-05767AF647
DIO3 Antibody [Alexa Fluor® 647]
Species: Hu, Mu, Rt, Ha, Rb
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DIO3 Antibody (NBP1-05767AF647). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a Dio3 peptide that I can inject in vivo to produce a biological effect. Can you provide information or confirm whether the Dio3 peptide you have would be effective as a biological agent in vivo?
    • I have pulled up information on the Dio3 peptides and recombinant proteins that we have available. Unfortunately I would not expect any of them to be biologically active and capable of producing a response when injected in vivo. The two peptides we carry are simply intended to be used for negative control competition assays to block signal of the primary and determine what is specific and non-specific as far as signal is concerned. The other two recombinant proteins are ones we distribute for a Taiwanese company called Abnova and they should not be biologically active based on the cell free wheat germ system employed to synthesize them. We will typically list our active proteins with an indication of such on our datasheet if it has been tested and usually the application of functional listed.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DIO3