D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-P, WB.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using NB100-56725	Applications	Species
Nishtala K, Phong TQ, Steil L et al. Proteomic analyses of age related changes in A.BY/SnJ mouse hearts. Proteome Sci [PMID: 23816347] (IHC-P, Mouse)	IHC-P	Mouse
Okamoto M, Koga S, Tatsuka M. Differential regulation of caspase-9 by ionizing radiation- and UV-induced apoptotic pathways in thymic cells. Mutat Res. 2010-06-01 [PMID: 20346366] (WB, Mouse) Details: D4-GDI (IMG-160A). WB: Mouse lymphoma 3SB cell line subject to either ionizing radiation or UV light, Fig 2D.	WB	Mouse
Hu LD, Zou HF, Zhan SX, Cao KM. Biphasic expression of RhoGDI2 in the progression of breast cancer and its negative relation with lymph node metastasis. Oncol Rep. 2007-06-01 [PMID: 17487395] (WB) Details: IHC (P): Fig 1 and Table I (human breast tumor and normal adjacent tissues (Fig 1 and Table 1); WB: Fig 2A (human breast cancer cell lines).	WB
Jenkins CE, Swiatoniowski A, Power MR, Lin TJ. Pseudomonas aeruginosa-induced human mast cell apoptosis is associated with up-regulation of endogenous Bcl-xS and down-regulation of Bcl-xL. J Immunol. 2006-12-01 [PMID: 17114473]
Wang Z, Zhang X, Shen P et al. A variant of estrogen receptor-{alpha}, hER-{alpha}36: transduction of estrogen- and antiestrogen-dependent membrane-initiated mitogenic signaling. Proc Natl Acad Sci U S A. 2006-06-13 [PMID: 16754886] (WB) Details: WB: HEk293 whole cell lysates (W), plasma membrane (M), cytosolic (C), and nuclear (N) fractions, Fig 3.	WB
Jenkins CE, Swiatoniowski A, Issekutz AC, Lin TJ. Pseudomonas aeruginosa exotoxin A induces human mast cell apoptosis by a caspase-8 and -3-dependent mechanism. J Biol Chem. 2004-08-27 [PMID: 15205454]
Liu CY, Lee CF, Hong CH, Wei YH. Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis. Ann N Y Acad Sci. 2004-04-01 [PMID: 15126291]
Ko CH, Shen SC, Chen YC. Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species. Free Radic Biol Med. 2004-04-01 [PMID: 15019974]
Chen YC, Shen SC, Lin HY. Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids. Biochem Pharmacol. 2003-10-01 [PMID: 14505793]
Shen SC, Chen YC, Hsu FL, Lee WR. Differential apoptosis-inducing effect of quercetin and its glycosides in human promyeloleukemic HL-60 cells by alternative activation of the caspase 3 cascade. J Cell Biochem. 2003-08-01 [PMID: 12874837]
Chen YC, Shen SC, Lee WR et al. Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production. Biochem Pharmacol. 2002-12-15 [PMID: 12445860]
Gildea JJ, Seraj MJ, Oxford G et al. RhoGDI2 is an invasion and metastasis suppressor gene in human cancer. Cancer Res. 2002-11-15 [PMID: 12438227]
Sato S, Fujita N, Tsuruo T. Interference with PDK1-Akt survival signaling pathway by UCN-01 (7-hydroxystaurosporine). Oncogene. 2002-03-07 [PMID: 11896604]
Yamaguchi H, Paranawithana SR, Lee MW et al. Epothilone B analogue (BMS-247550)-mediated cytotoxicity through induction of Bax conformational change in human breast cancer cells. Cancer Res. 2002-01-15 [PMID: 11809697]
Yamaguchi H, Wang HG. The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change. Oncogene. 2001-11-22 [PMID: 11753656]
Cuddeback SM, Yamaguchi H, Komatsu K et al. Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax. J Biol Chem. 2001-06-08 [PMID: 11259440]
Show All 16 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Human	07/18/2018	View Summary Application Western Blot Sample Tested HT-29 human colon adenocarcinoma cell line Species Human Lot AB021110A-04 Comments Comments HT-29 cells were treated with etoposide (100 ug/ml) for indicated time points. Total cell lysates were subjected to western blot. PVDF membrane were probed with 2 um/ml D4-GDI/RhoGDI2 (cleavage specific) Antibody (NB100-56725). A specific band was detected for cleaved RhoGDI2 at approximately 23 kDa. This experiment was conducted under reducing conditions.

Array NB100-56725

• D4-GDI/RhoGDI2 Antibodies
• D4-GDI/RhoGDI2 ELISA Kits
• D4-GDI/RhoGDI2 Lysate
• D4-GDI/RhoGDI2 Proteins