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LMOD1 Antibody

Images

 
Western Blot: LMOD1 Antibody [NBP1-89396] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane ...read more
Immunocytochemistry/ Immunofluorescence: LMOD1 Antibody [NBP1-89396] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LMOD1 Antibody [NBP1-89396] - Staining of human smooth muscle.
Immunohistochemistry-Paraffin: LMOD1 Antibody [NBP1-89396] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: LMOD1 Antibody [NBP1-89396] - Staining of human prostate shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: LMOD1 Antibody [NBP1-89396] - Staining in human prostate and liver tissues using anti-LMOD1 antibody. Corresponding LMOD1 RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: LMOD1 Antibody [NBP1-89396] - Staining of human colon.
Independent Antibodies: Staining of human liver, pancreas, prostate and tonsil using NBP1-89396 (A) shows similar protein distribution across tissues to independent antibody NBP1-89397 (B).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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LMOD1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TEKQSTGVYNREAMLNFCEKETKKLMQREMSMDESKQVETKTDAKNGEERGRDASKKALGPRRDSDLGKEPK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LMOD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
LMOD1 Protein (NBP1-89396PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LMOD1 Antibody

  • 64 kDa autoantigen 1D
  • 64 kDa autoantigen 1D3,1D
  • 64 kDa autoantigen D1,64kD
  • D1
  • FLJ55689
  • leimodin 1 (smooth muscle)
  • leiomodin 1 (smooth muscle)
  • Leiomodin, muscle form
  • leiomodin-1
  • SM-LMOD
  • Smooth muscle leiomodin
  • thyroid and eye muscle autoantigen D1 (64kD)
  • Thyroid-associated ophthalmopathy autoantigen

Background

LMOD1, also known as Leiomodin-1, has a 600 amino acid long isoform that is 67 kDa and a short 572 amino acid that is 64 kDa, belongs to the tropomodulin family, most commonly found in smooth muscle (heart, skeletal muscle, colon and small intestine), and has a putative membrane-spanning region and 2 types of tandemly repeated blocks. Current research is being performed on several diseases and disorders including thyroiditis, autoimmune thyroiditis, eye disease, graves' disease, hyperthyroidism, goiter, adenoma, breast cancer, retinoblastoma, carcinoma, thyroid hormone metabolism, mccune albright syndrome, bullous pemphigoid thyrotoxicosis, papillary thyroid carcinoma, coronary restenosis, follicular thyroid carcinoma, iodine deficiency, systemic lupus erythematosus, and pituitary adenom Interactions with this protein have been shown to involve ACTB, MYH11, TLN1, TPM1 and VCL proteins in smooth muscle contraction and muscle contraction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional LMOD1 Products

Array NBP1-89396

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Bioinformatics

Gene Symbol LMOD1