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DDX56 Antibody (4C5)

Images

 
Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in PC-12 (Cat # L012V1).
Immunocytochemistry/ Immunofluorescence: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of monoclonal antibody to DDX56 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml
Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in HeLa (Cat # L013V1).
Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody (M05), clone 4C5. Lane 1: DDX56 transfected lysatE (62 KDa). Lane 2: ...read more
Sandwich ELISA: DDX56 Antibody (4C5) [H00054606-M05] - Detection limit for recombinant GST tagged DDX56 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DDX56 Antibody (4C5) Summary

Immunogen
DDX56 (NP_061955, 450 a.a. ~ 547 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKFRPTAKPS
Specificity
DDX56 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDX56
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against recominant protein, transfected lysate and cell lysate for Western Blot. Has also been used for immunofluorescence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publication using
H00054606-M05 in the following applications:

Reactivity Notes

Human & Rat. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DDX56 Antibody (4C5)

  • ATP-dependent 61 kDa nucleolar RNA helicase
  • DDX26
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
  • DEAD box protein 21
  • DEAD box protein 56
  • DEAD-box RNA helicase
  • EC 3.6.1
  • EC 3.6.4.13,61-kd nucleolar helicase
  • NOH61DDX21
  • probable ATP-dependent RNA helicase DDX56
  • putative nucleolar RNA helicase

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDX56 Antibody (H00054606-M05)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


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IF/IHC
(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DDX56 Antibody (H00054606-M05) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDX56
Entrez
OMIM
Uniprot