Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in PC-12 (Cat # L012V1).
Immunocytochemistry/ Immunofluorescence: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of monoclonal antibody to DDX56 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml
Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in HeLa (Cat # L013V1).
Western Blot: DDX56 Antibody (4C5) [H00054606-M05] - Analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody (M05), clone 4C5. Lane 1: DDX56 transfected lysatE (62 KDa). Lane 2: ...read more
Sandwich ELISA: DDX56 Antibody (4C5) [H00054606-M05] - Detection limit for recombinant GST tagged DDX56 is approximately 0.1ng/ml as a capture antibody.
DDX56 (NP_061955, 450 a.a. ~ 547 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKFRPTAKPS
Specificity
DDX56 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDX56
Purity
IgG purified
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Antibody reactive against recominant protein, transfected lysate and cell lysate for Western Blot. Has also been used for immunofluorescence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publication using H00054606-M05 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for DDX56 Antibody (4C5)
ATP-dependent 61 kDa nucleolar RNA helicase
DDX26
DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
DEAD box protein 21
DEAD box protein 56
DEAD-box RNA helicase
EC 3.6.1
EC 3.6.4.13,61-kd nucleolar helicase
NOH61DDX21
probable ATP-dependent RNA helicase DDX56
putative nucleolar RNA helicase
Background
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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