![Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Western-Blot-NBP2-24529-img0001.jpg "Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.")
| Reactivity | Hu, Mu, Ca, Ch, Pm, Rt, Bv, Eq, Pm, Ma-Op, RMSpecies Glossary |
| Applications | WB, Simple Western, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | A portion of amino acids 50-100 of human eIF4A2 was used as the immunogen for the antibody. |
| Specificity | The immunogen for this antibody has 100% homology to eIF4A1 and 89% homology to eIF4A3. Specificity to eIF4A2 has not been determined. |
| Predicted Species | Rat (100%), Equine (100%), Monkey (100%), Opossum (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EIF4A2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 0.2 ml PBS and 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-24529 | Applications | Species |
|---|---|---|
| Shah A, Bhandari R IP6K1 upregulates the formation of processing bodies by promoting proteome remodeling on the mRNA cap bioRxiv 2020-07-14 [PMID: 34841428] (WB, ICC/IF, Human) | WB, ICC/IF | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Verified Customer |
WB | Mouse | 02/18/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for eIF4A2 Antibody (NBP2-24529)Find related products by research area.
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![Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Immunohistochemistry-Paraffin-NBP2-24529-img0002.jpg "Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.")
![Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Simple-Western-NBP2-24529-img0003.jpg "Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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