![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0010.jpg "Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.")
| Reactivity | Hu, Mu, Rt, Ha, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | 3B9E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human DDX3 (AA: 518-661) expressed in E. Coli. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DDX3X |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody is Cytof ready. Western blot reported from a verified customer review. |
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| Theoretical MW | 73.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Publication using NBP2-52457 | Applications | Species |
|---|---|---|
| Sun X, Wang S, Lin X et al. Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells. Proteomics 2017-06-20 [PMID: 28516729] (WB, Hamster) | WB | Hamster |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Verified Customer |
WB | Human | 06/21/2018 |
Summary
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Enlarge |
reviewed by:
Verified Customer |
WB | the baby hamster kidney cell line (BHK-21) | 12/13/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for DDX3 Antibody (NBP2-52457)Find related products by research area.
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| Verified Customer 06/21/2018 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 12/13/2017 |
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| Application: | WB | |
| Species: | the baby hamster kidney cell line (BHK-21) |
| Gene Symbol | DDX3X |
![Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunohistochemistry-NBP2-52457-img0006.jpg "Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.")
![Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Flow-Cytometry-NBP2-52457-img0002.jpg "Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).")
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0007.jpg "Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.")
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against HEK293 (1) and DDX3X (AA: 518-661)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0009.jpg "Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against HEK293 (1) and DDX3X (AA: 518-661)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0008.jpg "Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)")
![Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of paraffin-embedded cervical cancer tissues using DDX3X mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunohistochemistry-NBP2-52457-img0005.jpg "Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of paraffin-embedded cervical cancer tissues using DDX3X mouse mAb with DAB staining.")
![Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Flow-Cytometry-NBP2-52457-img0001.jpg "Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).")
![Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunofluorescence-NBP2-52457-img0003.jpg "Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
![Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunofluorescence-NBP2-52457-img0004.jpg "Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
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