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NIPP1 Antibody

Images

 
Genetic Strategies: Western Blot: NIPP1 Antibody [NBP1-90113] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Independent Antibodies: Western Blot: NIPP1 Antibody [NBP1-90113] - Analysis using Anti-PPP1R8 antibody NBP1-90113 (A) shows similar pattern to independent antibody NBP1-90112 (B).
Independent Antibodies: Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90113] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-PPP1R8 antibody NBP1-90113 (A) shows similar protein ...read more
Immunocytochemistry/ Immunofluorescence: NIPP1 Antibody [NBP1-90113] - Staining of human cell line A-431 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90113] - Staining of human cerebral cortex.
Western Blot: NIPP1 Antibody [NBP1-90113] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90113] - Staining of human testis.
Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90113] - Staining of human lymph node.
Immunohistochemistry-Paraffin: NIPP1 Antibody [NBP1-90113] - Staining of human colon.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NIPP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP1R8
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NIPP1 Protein (NBP1-90113PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NIPP1 Antibody

  • activator of RNA decay
  • ARD1
  • ard-1
  • ARD1nuclear inhibitor of protein phosphatase 1
  • NIPP1
  • NIPP1nuclear inhibitor of protein phosphatase-1
  • NIPP-1nuclear subunit of PP-1
  • PPP1R8
  • PRO2047
  • Protein phosphatase 1 regulatory inhibitor subunit 8
  • protein phosphatase 1 regulatory subunit 8
  • protein phosphatase 1, regulatory (inhibitor) subunit 8
  • RNase E

Background

NIPP1, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPP1R8