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PNPT1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: PNPT1 Antibody [NBP1-82485] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & mitochondria. Antibody staining shown in green.
Immunohistochemistry-Paraffin: PNPT1 Antibody [NBP1-82485] - Staining of human rectum shows strong cytoplasmic positivity with a granular pattern in glandular cells.
Independent Antibodies: Analysis using NBP1-82485 (A) shows similar pattern to independent antibody NBP2-38211 (B).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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PNPT1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPA
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PNPT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PNPT1 Protein (NBP1-82485PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PNPT1 Antibody

  • DKFZp762K1914
  • EC 2.7.7
  • EC 2.7.7.8
  • mitochondrial
  • OLD35
  • old-35
  • PNPase 1
  • PNPase old-35
  • PNPase
  • Polynucleotide phosphorylase 1
  • Polynucleotide phosphorylase-like protein
  • polyribonucleotide nucleotidyltransferase 1

Background

PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PNPT1 Antibody (NBP1-82485) (0)

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Secondary Antibodies

 

Isotype Controls

Additional PNPT1 Products

Array NBP1-82485

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Bioinformatics

Gene Symbol PNPT1