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DDX5 Antibody

Images

 
Genetic Strategies: Western Blot: DDX5 Antibody [NBP1-83406] - Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. ...read more
Immunocytochemistry/ Immunofluorescence: DDX5 Antibody [NBP1-83406] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.
Western Blot: DDX5 Antibody [NBP1-83406] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.
Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human endometrium shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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DDX5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 -1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DDX5 Protein (NBP1-83406PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DDX5 Antibody

  • B-cell CLL/lymphoma 7 protein family member C
  • B-cell CLL/lymphoma 7C
  • DDX5
  • DEAD/H
  • G17P1
  • HELR
  • HLR1
  • HUMP68

Background

DDX5, also known as Probable ATP-dependent RNA helicase DDX5, is a 69.1 kDa, 613 amino acid protein that regulates pre-mRNA splicing by increasing the inclusion of tau exon 10. Current research with the protein is being conducted for diseases and disorders such as squamous cell carcinoma, breast cancer, fibrosis, prostate cancer, hepatitis, malaria, and myeloma. The protein interacts in translational control and spliceosome pathways with HIST1H4A- HIST1H4E proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DDX5