Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
A431 human epithelial carcinoma cell line was stained with Human CD109 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4385, filled histogram) or isotype control antibody (Catalog # 5-001-A, ...read more
CD109 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using 15 µg/mL Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4385) overnight at ...read more
Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD109 at approximately 161 kDa (as indicated) using 20 µg/mL ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CD109 Antibody [Unconjugated]
150 kDa TGF-beta-1-binding protein
activated T-cell marker CD109
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7
CD109 antigen (Gov platelet alloantigens)
CD109 antigen
CD109 molecule
CD109
CPAMD7
CPAMD7r150
DKFZp762L1111
FLJ38569
FLJ41966
Gov platelet alloantigens
p180
Platelet-specific Gov antigen
RP11-525G3.1
Background
CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares 71-73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5-9). It is produced as a 170-180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).
Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
Lin, M. et al. (2002) Blood 99:1683.
Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
Hashimoto, M. et al. (2004) Oncogene 23:3716.
Solomon, K.R. et al. (2004) Gene 327:171.
Finnson, K.W. et al. (2006) FASEB J. 20:1525.
Schuh, A.C. et al. (2002) Blood 99:1692.
Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
Rozman, P. (2002) Transpl. Immunol. 10:165.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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