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![Western Blot: DDX5 Antibody [NB200-351] - Detection of Human and Mouse DDX5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody NB200-351 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NB200-351-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: DDX5 Antibody [NB200-351] - Detection of Human and Mouse DDX5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody NB200-351 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: DDX5 Antibody [NB200-351] - Detection of human DDX5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DDX5 NB200-351 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-NB200-351-img0009.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DDX5 Antibody [NB200-351] - Detection of DDX5 in whole cell lysates from HeLa (5, 15, 50 ug), 293 (T; 50 ug), and MCF7 (M; 50 ug), and K562 (K; 50 ug) for WB (A) and HeLa (1 mg) for IP (B). Antibodies: NB 200-351 used at 0.04 ug/ml for WB (A & B) and at 3 ug/ml lysate for IP (B). DDX5 was also immonuprecipitated with NB 200-350 used at 3 ug/ml lysate. Detection: ECL 30 seconds.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NB200-351-img0001.jpg)
![Immunohistochemistry: DDX5 Antibody [NB200-351] - ~ ~Sample: FFPE section of human breast carcinoma.~ Antibody: Affinity purified rabbit anti-DDX5 ~Cat. No. Lot2 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-NB200-351-img0003.jpg)
![Immunohistochemistry: DDX5 Antibody [NB200-351] - ~~ Sample: FFPE section of mouse renal cell carcinoma.~ Antibody: Affinity purified rabbit anti-DDX5 ~Cat. No. Lot2 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-NB200-351-img0004.jpg)
![Immunohistochemistry: DDX5 Antibody [NB200-351] - Detection of mouse DDX5 by immunohistochemistry. Sample: FFPE section of mouse skin. Antibody: Affinity purified rabbit anti-DDX5 NB200-351 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-NB200-351-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DDX5 Antibody [NB200-351] - Detection of human DDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX5 antibody NB200-351 (lot NB200-351-2) used for IP at 6 ug per reaction. DDX5 was also immunoprecipitated by a previous lot of this antibody (lot NB200-351-1) and another rabbit anti-DDX5 antibody. For blotting immunoprecipitated DDX5, NB200-351 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunoprecipitation-NB200-351-img0007.jpg)
![Western Blot: DDX5 Antibody [NBP1-83406] - Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP1-83406-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DDX5 Antibody [NBP1-83406] - Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP1-83406] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83406-img0010.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0017.jpg)
![Western Blot: DDX5 Antibody [NBP1-83406] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP1-83406-img0009.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0013.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0014.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0015.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-83406] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-83406-img0016.jpg)
![Western Blot: DDX5 Antibody (1K6X10) [NBP3-15371] - Western blot analysis of extracts of various cell lines, using DDX5 Rabbit mAb (NBP3-15371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Western-Blot-NBP3-15371-img0007.jpg "Western Blot: DDX5 Antibody (1K6X10) [NBP3-15371] - Western blot analysis of extracts of various cell lines, using DDX5 Rabbit mAb (NBP3-15371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunofluorescence analysis of NIH-3T3 cells using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunocytochemistry-Immunofluorescence-NBP3-15371-img0006.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunohistochemistry of paraffin-embedded mouse spinal cord using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunohistochemistry-Paraffin-NBP3-15371-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunofluorescence analysis of C6 cells using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunocytochemistry-Immunofluorescence-NBP3-15371-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunofluorescence analysis of HeLa cells using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunocytochemistry-Immunofluorescence-NBP3-15371-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunohistochemistry of paraffin-embedded rat ovary using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunohistochemistry-Paraffin-NBP3-15371-img0001.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunohistochemistry of paraffin-embedded human esophageal using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunohistochemistry-Paraffin-NBP3-15371-img0002.jpg)
![Immunoprecipitation: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunoprecipitation analysis of 600ug extracts of Mouse testis using 3ug DDX5 antibody (NBP3-15371). Western blot was performed from the immunoprecipitate using DDX5 (NBP3-15371) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/DDX5-Antibody-1K6X10-Immunoprecipitation-NBP3-15371-img0008.jpg)
![Western Blot: DDX5 Antibody [NBP1-30944] - Human lymphocytes. Antibody 1:500 dilution in milk. 6 minute exposure. ~25kDA band shows up as strong as the expected band at ~75kDA. This image was submitted via customer review.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP1-30944-img0006.jpg "Western Blot: DDX5 Antibody [NBP1-30944] - Human lymphocytes. Antibody 1:500 dilution in milk. 6 minute exposure. ~25kDA band shows up as strong as the expected band at ~75kDA. This image was submitted via customer review.")
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP1-30944] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30944-img0003.jpg)
![Immunohistochemistry-Paraffin: DDX5 Antibody [NBP1-30944] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NBP1-30944-img0004.jpg)
![Western Blot: DDX5 Antibody [NBP1-30944] - 30 ug of whole cell lysate, H1299 7. 5% SDS-PAGE DDX5. Antibody at 1:1000.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP1-30944-img0005.jpg)
![Western Blot: DDX5 Antibody [NB100-1410] - (0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NB100-1410-img0006.jpg "Western Blot: DDX5 Antibody [NB100-1410] - (0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DDX5 Antibody [NB100-1410] - Paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained using a previous batch.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunohistochemistry-Paraffin-NB100-1410-img0004.jpg)
![Western Blot: DDX5 Antibody [NB100-1410] - DDX5 / p68 RNA helicase in Hela lysate using this antibody at 0.5 ug/ ml.](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NB100-1410-img0005.jpg)
![Immunohistochemistry: DDX5 Antibody (2C3) [NBP3-26627] - Image of DDX5 Antibody (2C3) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26627_rabbit-ddx5-mab-2c3-26220241422520.jpg "Immunohistochemistry: DDX5 Antibody (2C3) [NBP3-26627] - Image of DDX5 Antibody (2C3) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: DDX5 Antibody (2C3) [NBP3-26627] - Positive Western Blot detected in: HepG2 whole cell lysate, HT-29 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue. All lanes: DDX5 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70, 61 kDa Observed band size: 70 kDa](http://images.novusbio.com/fullsize/nbp3-26627_rabbit-ddx5-mab-2c3-282202411413612.jpg)
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody (2C3) [NBP3-26627] - Staining of HepG2 Cells with DDX5 Antibody (2C3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26627_rabbit-ddx5-mab-2c3-28220241323637.jpg)
![Flow Cytometry: DDX5 Antibody (2C3) [NBP3-26627] - Overlay histogram showing Hela cells stained with DDX5 Antibody (2C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26627_rabbit-ddx5-mab-2c3-282202414183613.jpg)
![Western Blot: DDX5 Antibody [NBP2-98889] - Anti-DDX5 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP2-98889-img0003.jpg "Western Blot: DDX5 Antibody [NBP2-98889] - Anti-DDX5 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 70 kDa")
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP2-98889] - Immunofluorescence staining of DDX5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DDX5 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98889-img0002.jpg)
![Immunoprecipitation: DDX5 Antibody [NBP2-98889] - DDX5 was immunoprecipitated using: Lane A: 0.5 mg PC-3 Whole Cell Lysate4 ul anti-DDX5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DDX5 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunoprecipitation-NBP2-98889-img0001.jpg)
![Western Blot: DDX5 Antibody [NBP3-17654] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/DDX5-Antibody-Western-Blot-NBP3-17654-img0002.jpg "Western Blot: DDX5 Antibody [NBP3-17654] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunocytochemistry/Immunofluorescence: DDX5 Antibody [NBP3-17654] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.](http://images.novusbio.com/fullsize/DDX5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17654-img0001.jpg)
![Western Blot: DDX5 Overexpression Lysate (Adult Normal) [NBL1-09801] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX5.](http://images.novusbio.com/fullsize/DDX5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09801-img0002.jpg "Western Blot: DDX5 Overexpression Lysate (Adult Normal) [NBL1-09801] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX5.")
![ELISA: Human DDX5 ELISA Kit (Colorimetric) [NBP3-27340] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27340_human-ddx5-elisa-kit-colorimetric-1142024103124.png "ELISA: Human DDX5 ELISA Kit (Colorimetric) [NBP3-27340] - Standard Curve Reference")
![ELISA: Human DDX5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31347] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31347_human-ddx5-ready-to-use-elisa-kit-colorimetric-2062024153394.png "ELISA: Human DDX5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31347] - Standard Curve Reference")