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EIF3A Antibody

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Independent Antibodies: Western Blot: EIF3A Antibody [NBP1-18891] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. eIF3A/EIF3S10 was also immunoprecipitated by rabbit antieIF3A/ EIF3S10 antibody ...read more
TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation ...read more
TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Xp, ZeSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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EIF3A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human translation initiation factor 3 subunit A (eukaryotic translation initiation factor 3, subunit 10) using the numbering given in entry NP_003741.1 (GeneID 8661).
Predicted Species
Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Reviewed Applications
Read 1 Review rated 5
using
NBP1-18891 in the following applications:

Publications
Read Publications using
NBP1-18891 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF3A Antibody

  • centrosomin homolog
  • cytoplasmic protein p167
  • eIF3 p167
  • eIF3 p180
  • eIF3 p185
  • EIF3, p180 subunit
  • eIF3a
  • eIF3-p170
  • EIF3S10EIF3
  • eIF-3-theta
  • eIF3-theta
  • Eukaryotic translation initiation factor 3 subunit 10
  • eukaryotic translation initiation factor 3 subunit A
  • eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD)
  • eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD)
  • eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa
  • eukaryotic translation initiation factor 3, subunit 10, 170kD
  • eukaryotic translation initiation factor 3, subunit A
  • KIAA0139P167
  • p180
  • p185
  • TIF32

Background

Eukaryotic initiation factor 3 subunit A (eIF3A) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5-end of cellular mRNAs near the cap structure (m7GpppN). eIF3A is also known as EIF3S10 (eukaryotic initiation factor 3 subunit 7). eIF3A is the largest subunit of the eIF3 complex. eIF3A expression is regulated through the cell cycle. It's expression peaks in S-phase and it is proposed to play a role in regulating the translation of mRNAs involved in the regulation of cell cycle progression and proliferation. Alternative names for eIF3A include eIF-3-theta, eIF-3 p167, eIF-3 p170, eIF-3 p180, eIF3-p185, EIF3S10, and KIAA0139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EIF3A Antibody (NBP1-18891)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: WB, Western Blot.


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WB
(7)
Western Blot
(1)
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Human
(7)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-18891 Applications Species
Kejiou NS, Ilan L, Aigner S et al. Pyruvate Kinase M (PKM) binds ribosomes in a poly-ADP ribosylation dependent manner to induce translational stalling Nucleic acids research 2023-05-24 [PMID: 37224531] (Western Blot, Human) Western Blot Human
Duan H, Zhang S, Zarai Y et al. eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content The EMBO Journal 2023-06-15 [PMID: 37155573]
Zhao Y, Zhao H, Zhang D et al. YTHDF3 Facilitates eIF2AK2 and eIF3A Recruitment on mRNAs to Regulate Translational Processes in Oxaliplatin-Resistant Colorectal Cancer ACS chemical biology 2022-06-15 [PMID: 35708211]
Gong C, Krupka JA, Gao J Et al. Sequential inverse dysregulation of the RNA helicases DDX3X and DDX3Y facilitates MYC-driven lymphomagenesis Molecular cell 2021-08-18 [PMID: 34437837] (WB, Human) WB Human
Hassan Hayek, Lauriane Gross, Aurélie Janvier, Laure Schaeffer, Franck Martin, Gilbert Eriani, Christine Allmang eIF3 interacts with histone H4 messenger RNA to regulate its translation The Journal of Biological Chemistry 2021-03-23 [PMID: 33766559]
Jeong SJ, Park S, Nguyen LT et al. A threonyl-tRNA synthetase-mediated translation initiation machinery Nat Commun 2019-03-22 [PMID: 30902983] (WB, Human) WB Human
Toompuu M, Karblane K, Pata P et al. ABCE1 is essential for S phase progression in human cells. Cell Cycle. 2016-03-17 [PMID: 26985706] (WB, Human) WB Human
Lee AS, Kranzusch PJ, Cate JH. eIF3 targets cell-proliferation messenger RNAs for translational activation or repression Nature 2015-04-06 [PMID: 25849773] (WB, Human) WB Human
Chazal PE, Daguenet E, Wendling C et al. EJC core component MLN51 interacts with eIF3 and activates translation Proc Natl Acad Sci U S A 2013-03-25 [PMID: 23530232] (WB, Human) WB Human
Lin Y, Li F, Huang L et al. eIF3 promotes early translation elongation to ensure mitochondrial homeostasis and skeletal muscle health bioRxiv 1905-07-11 (WB, Human) WB Human
Show All 11 Publications.

Review for EIF3A Antibody (NBP1-18891) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-18891:
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WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot EIF3A NBP1-18891
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5
reviewed by:
Verified Customer
WB Other 06/17/2016
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Summary

ApplicationWestern Blot
Sample TestedXenopus brain lysate
SpeciesOther

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Secondary Antibodies

 

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Verified Customer
06/17/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol EIF3A
Entrez
Uniprot