![Western Blot: DAZL Antibody (DAZL/4253) - Azide and BSA Free [NBP3-08742] - Western blot Analysis testis tissue lysate using DAZL Mouse Monoclonal Antibody (DAZL/4253).](http://images.novusbio.com/fullsize/DAZL-Antibody-DAZL-4253-Azide-and-BSA-Free-Western-Blot-NBP3-08742-img0002.jpg "Western Blot: DAZL Antibody (DAZL/4253) - Azide and BSA Free [NBP3-08742] - Western blot Analysis testis tissue lysate using DAZL Mouse Monoclonal Antibody (DAZL/4253).")
| Reactivity | Hu, Mu, Rt, Pm-CmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | DAZL/4253 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07840). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV. (Uniprot: Q92904) |
| Localization | Cytoplasmic |
| Specificity | Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DAZL |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DAZL |
![Immunohistochemistry-Paraffin: DAZL Antibody (DAZL/4253) - Azide and BSA Free [NBP3-08742] - Formalin-fixed, paraffin-embedded human testis stained with DAZL Mouse Monoclonal Antibody (DAZL/4253).](http://images.novusbio.com/fullsize/DAZL-Antibody-DAZL-4253-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08742-img0001.jpg "Immunohistochemistry-Paraffin: DAZL Antibody (DAZL/4253) - Azide and BSA Free [NBP3-08742] - Formalin-fixed, paraffin-embedded human testis stained with DAZL Mouse Monoclonal Antibody (DAZL/4253).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")