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CPEB1 Antibody

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Western Blot: CPEB1 Antibody [NB100-1437] - Staining of human brain lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 0.5 ug/ml. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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CPEB1 Antibody Summary

Immunogen
Peptide with sequence C-PLMRNQKNRDSS corresponding to C-Terminus according to NP_085097.3, NP_001073001.1, NP_001073002.1, NP_001073003.1.
Localization
Cytoplasmic
Specificity
This antibody is expected to recognise alle reported isoforms. Variants (NP_001073002.1; NP_001073003.1) encode the same isoform.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CPEB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.5 - 2 ug/ml
Application Notes
WB: Approx. 65-70 kDa band observed in human brain lysates (predicted MW of 65 kDa band according to NP_085097). Please note this band was always accompanied by a slightly stronger band at approx. 48 kDa and both bands could be blocked by incubation with the immunizing peptide.
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
CPEB1 Overexpression Lysate
Publications
Read Publications using
NB100-1437 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CPEB1 Antibody

  • CPEBCEBP
  • CPE-binding protein 1
  • CPE-BP1
  • cytoplasmic polyadenylation element binding protein 1
  • cytoplasmic polyadenylation element-binding protein 1
  • FLJ13203
  • h-CEBP
  • hCPEB-1
  • MGC34136
  • MGC60106

Background

This gene encodes a member of the cytoplasmic polyadenylation element (CPE) binding protein family. This highly conserved protein binds to a specific RNA sequence called the CPE found in the 3' UTR of some mRNAs. Similar proteins in Xenopus and mouse function to induce cytoplasmic polyadenylation of dormant mRNAs with short polyA tails, resulting in their translation. Members of this protein family regulate translation of cyclin B1 during embryonic cell divisions. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CPEB1 Antibody (NB100-1437)(3)

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Video Protocols

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Bioinformatics

Gene Symbol CPEB1
Entrez
Uniprot