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Boll Antibody

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Western Blot: Boll Antibody [NBP2-33538] - Analysis in control (vector only transfected HEK293T lysate) and BOLL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human lymph node.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human testis shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining in human testis and kidney tissues using anti-BOLL antibody. Corresponding BOLL RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human colon, liver, lymph node and testis using Anti-BOLL antibody NBP2-33538 (A) shows similar protein distribution ...read more
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human liver.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human colon.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Boll Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: VTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQ
Predicted Species
Mouse (94%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BOLL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Boll Protein (NBP2-33538PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Boll Antibody

  • bol, boule-like (Drosophila)
  • BOULEbol (Drosophila boule homolog)-like
  • protein boule-like

Background

Boll belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BOLL
Uniprot