![Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Sections of the ectopic lesions collected from E2 or E2 plus P4-treated recipients (D16, n = 6) were subjected to histological examination. (B) Representative images (20X) showing IHC analysis using antibodies against an angiogenetic regulator CCN1. The immunostaining intensity of CCN1 was analyzed by ImageJ software. The numerical values were analyzed by One-way ANOVA followed by Dunnett's post hoc test and expressed as mean +/- SEM. Statistical significance is defined as *: p<0.01. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0165347) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Immunohistochemistry-NB100-356-img0007.jpg "Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Sections of the ectopic lesions collected from E2 or E2 plus P4-treated recipients (D16, n = 6) were subjected to histological examination. (B) Representative images (20X) showing IHC analysis using antibodies against an angiogenetic regulator CCN1. The immunostaining intensity of CCN1 was analyzed by ImageJ software. The numerical values were analyzed by One-way ANOVA followed by Dunnett's post hoc test and expressed as mean +/- SEM. Statistical significance is defined as *: p<0.01. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0165347) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RbSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, B/N |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A synthetic peptide made to the human CYR61 protein sequence (between residues 150-250). [UniProt# O00622] |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CCN1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In Western blot a band is observed at ~37 kDa, representing the processed form of CYR61 protein. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. |
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| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
ICC | Other | 12/12/2014 |
Summary
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Enlarge |
reviewed by:
chandra kurapaty |
WB | Human | 06/18/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cyr61/CCN1 Antibody (NB100-356)Find related products by research area.
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| Verified Customer 12/12/2014 |
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| Application: | ICC | |
| Species: | Other |
| chandra kurapaty 06/18/2014 |
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| Application: | WB | |
| Species: | Human |
![Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Total protein from Human liver, spleen and HepG2 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-CYR61 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the low expression level of CYR61 in HepG2 cells.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Western-Blot-NB100-356-img0006.jpg "Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Total protein from Human liver, spleen and HepG2 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-CYR61 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the low expression level of CYR61 in HepG2 cells.")
![Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody [NB100-356] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Cyr61/CCN1 (NB100-356) at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-356-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody [NB100-356] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Cyr61/CCN1 (NB100-356) at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate using NB 100-356. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Western-Blot-NB100-356-img0004.jpg "Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate using NB 100-356. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.")
![Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Immunohistochemistry-NB100-356-img0001.jpg "Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.")
![Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Immunohistochemistry-NB100-356-img0002.jpg "Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.")
![Simple Western: Cyr61/CCN1 Antibody [NB100-356] - Image shows a specific band for CYR61 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Simple-Western-NB100-356-img0003.jpg "Simple Western: Cyr61/CCN1 Antibody [NB100-356] - Image shows a specific band for CYR61 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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