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CXCR5 Antibody (9Q10I2)

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Western Blot: CXCR5 Antibody (9Q10I2) [NBP3-16723] - Western blot analysis of extracts of various cell lines, using CXCR5 Rabbit mAb (NBP3-16723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence:CXCR5 Antibody (9Q10I2) [NBP3-16723] - Confocal imaging of human tonsil using CXCR5 Rabbit mAb (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: ...read more
Immunocytochemistry/ Immunofluorescence: CXCR5 Antibody (9Q10I2) [NBP3-16723] - Confocal imaging of human tonsil using CXCR5 Rabbit mAb. DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure ...read more
Immunocytochemistry/ Immunofluorescence: CXCR5 Antibody (9Q10I2) [CXCR5] - Confocal imaging of human tonsil using CXCR5 Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
9Q10I2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CXCR5 Antibody (9Q10I2) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 273-372 of human CXCR5 (P32302). SPYHIVIFLDTLARLKAVDNTCKLNGSLPVAITMCEFLGLAHCCLNPMLYTFAGVKFRSDLSRLLTKLGCTGPASLCQLFPSWRRSSLSESENATSLTTF
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CXCR5
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CXCR5 Antibody (9Q10I2)

  • BLR1
  • BLR1MDR-15
  • Burkitt lymphoma receptor 1
  • Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
  • Burkitt lymphoma receptor 1, GTP-binding protein
  • CD185 antigen
  • CD185
  • chemokine (C-X-C motif) receptor 5
  • C-X-C chemokine receptor type 5
  • CXCR5
  • CXC-R5
  • MDR15CXCR-5
  • MGC117347
  • Monocyte-derived receptor 15

Background

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CXCR5