Regarding recommended dilutions the manual contains: "Immunohistochemistry-Paraffin, Western Blot 1-3 ug/ml" Does it mean 1-3 ug/ml is recommended for both applications or there is simply no recommended dilution IHC-P?

Name: CXCR3 Antibody
Brand: Novus Biologicals
SKU: NB100-56404
Rating: 5 (2 reviews)

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Accepted Answer

For our product NB100-56404, we did not have a dilution recommendation listed for IHC on our datasheet. I am sorry for any confusion. This application was added to our product based on a publication that actually uses this product at 1:200 in paraformaldehyde fixed frozen tissue samples: NCBI site.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, MuSpecies Glossary
Applications	WB, Func, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Immunogen	This antibody was developed against a synthetic peptide corresponding to amino acids 290-303 of human CXCR3.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	CXCR3
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein G purified

Publications using NB100-56404	Applications	Species
Alluri SR, Higashi Y, Berendzen A et al. Synthesis and preclinical evaluation of a novel fluorine-18 labeled small-molecule PET radiotracer for imaging of CXCR3 receptor in mouse models of atherosclerosis EJNMMI Research 2023-07-13 [PMID: 37438543] (Immunohistochemistry)	Immunohistochemistry
Alluri SR, Higashi Y, Berendzen A et al. Synthesis and preclinical evaluation of a novel fluorine-18 labeled small-molecule PET radiotracer for imaging of CXCR3 receptor in mouse models of atherosclerosis Research square 2023-02-23 [PMID: 36865232] (IHC, Mouse)	IHC	Mouse
Zhu L, Gao H, Huang S et al. Combination of STING agonist and CXCR3 antagonist disrupts immune tolerance to overcome anti-PD-L1 resistance in lung adenocarcinoma under oxidative stress Gene 2022-10-19 [PMID: 36272651] (IF/IHC, Mouse)	IF/IHC	Mouse
Lin Y, Zhou HC, Chen N et al. Unveiling the improved targeting migration of mesenchymal stem cells with CXC chemokine receptor 3-modification using intravital NIR-II photoacoustic imaging Journal of Nanobiotechnology 2022-12-01 [PMID: 35764961]
Park JY, Yoo KD, Bae E et al. Blockade of STAT3 signaling alleviates progression of acute kidney injury-to-chronic kidney disease through anti-apoptosis American journal of physiology. Renal physiology 2022-03-21 [PMID: 35311382] (IF/IHC)	IF/IHC
Javeed N, Her TK, Brown MR Et al. Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes Cell reports 2021-08-24 [PMID: 34433033] (PLA, Mouse)	PLA	Mouse
Petrisko TJ, Bloemer J, Pinky PD et al. Neuronal CXCL10/CXCR3 Axis Mediates the Induction of Cerebral Hyperexcitability by Peripheral Viral Challenge Front Neurosci 2020-03-24 [PMID: 32265633] (ICC/IF, Mouse)	ICC/IF	Mouse
Alvarez-Carbonell D, Ye F, Ramanath N et al. Cross-talk between microglia and neurons regulates HIV latency PLoS Pathog. 2019-12-01 [PMID: 31887215] (ICC/IF, Human)	ICC/IF	Human
Suttorp CM, Cremers NA, van Rheden R et al. Chemokine Signaling during Midline Epithelial Seam Disintegration Facilitates Palatal Fusion Front Cell Dev Biol. 2017-10-30 [PMID: 29164113] (IHC-P, Mouse) Details: Used CXCR3 IHC antibody from Novus to quantify CXCR3 in palatal shelve mesenchyme	IHC-P	Mouse
Brambilla R, Persaud T, Hu XC et al. Transgenic Inhibition of Astroglial NF-?B Improves Functional Outcome in Experimental Autoimmune Encephalomyelitis by Suppressing Chronic Central Nervous System Inflammation . J Immunol 182 2628-2640. 2009-01-01 [PMID: 19234157]

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Gene Symbol	CXCR3
Entrez	2833(Human) 12766(Mouse)
Uniprot	P49682()

	Verified Customer 01/18/2023
Application:	ICC
Species:	Mouse

	Verified Customer 09/11/2014
Application:	WB
Species:	Human