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Recombinant Human CXCL13/BLC/BCA-1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-34885-1mg
Carrier-Free
NBP2-34885-100ug
Carrier-Free
NBP2-34885-500ug
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Recombinant Human CXCL13/BLC/BCA-1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 87 amino acids corresponding to CXCL13/BLC/BCA-1 Source: E. coli

Uniprot ID: O43927

Amino Acid Sequence: VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Details of Functionality
CXCL13 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CXCL13
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of CXCL13/BLC/BCA-1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
10.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-34885.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human CXCL13/BLC/BCA-1 Protein

  • ANGIE
  • ANGIE2
  • B cell-attracting chemokine 1
  • B lymphocyte chemoattractant
  • BCA1
  • BCA-1
  • BCA-1/CXCL13
  • BCA1B-cell chemoattractant
  • BCA-1CXC chemokine BLC
  • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
  • BLC
  • BLCSmall-inducible cytokine B13
  • BLR1L
  • B-lymphocyte chemoattractant
  • chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
  • chemokine (C-X-C motif) ligand 13
  • C-X-C motif chemokine 13
  • CXCL13
  • SCYB13
  • SCYB13B-cell-attracting chemokine 1
  • small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

C-X-C motif chemokine 13 encoded by the CXCL13 gene located on human chromosome 4 is constitutively expressed in secondary lymphoid organs. It is also known as B lymphocyte chemoattractant (BLC). As its name suggests, CXCL13 is selectively chemotactic for B cells belonging to both the B-1 and B-2 subsets, and elicits its effects by interacting with chemokine receptor CXCR5. It and its receptor CXCR5 control the organization of B cells within follicles of lymphoid tissues. Recombinant human CXCL13 is a single non-glycosylated polypeptide chain containing 87 amino acids and mature human BCA-1 shares 64 % amino acid sequence similarity with the murine protein and 23-34 % amino acid sequence identity with other known CXC chemokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
CXCL13/BLC/BCA-1 Prot ...
NBP2-34885
Recombinant Human CXCL13/BLC/BCA-1 Protein
Species: Hu
Applications: PAGE, Bioactivity

Publications for CXCL13/BLC/BCA-1 Protein (NBP2-34885)(1)

We have publications tested in 1 application: Func.


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Bioinformatics

Gene Symbol CXCL13
Uniprot