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CX3CR1 Antibody - BSA Free

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Western Blot: CX3CR1 Antibody [NBP1-76872] - Analysis of CX3CR1 in THP-1 cell lysate in the absence (lane A) or presence (lane B) of blocking peptide with anti-CX3CR1 (EL) using NBP1-76872 at 1:500 dilution.
Western Blot: CX3CR1 Antibody [NBP1-76872] - Analysis of CX3CR1 in THP-1 cell lysate in the absence (lane A) or presence (lane B) of blocking peptide with anti-CX3CR1 (EL) using NBP1-76872 at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: CX3CR1 Antibody [NBP1-76872] - Immunofluorescence of CX3CR1 in THP-1 cells with CX3CR1 antibody at 20 ug/ml.
Immunohistochemistry-Paraffin: CX3CR1 Antibody [NBP1-76872] - IHC of CX3CR1 in human spleen using 10 ug/mL of NBP1-76872

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76872AF350
Alexa Fluor 405
NBP1-76872AF405
Alexa Fluor 488
NBP1-76872AF488
Alexa Fluor 532
NBP1-76872AF532
Alexa Fluor 594
NBP1-76872AF594
Alexa Fluor 647
NBP1-76872AF647
Alexa Fluor 700
NBP1-76872AF700
Alexa Fluor 750
NBP1-76872AF750
Biotin
NBP1-76872B
CoraFluor 1
NBP1-76872CL1
DyLight 350
NBP1-76872UV
DyLight 405
NBP1-76872V
DyLight 488
NBP1-76872G
DyLight 550
NBP1-76872R
DyLight 594
NBP1-76872DL594
DyLight 650
NBP1-76872C
DyLight 680
NBP1-76872FR
DyLight 755
NBP1-76872IR
FITC
NBP1-76872F
HRP
NBP1-76872H
Janelia Fluor 525
NBP1-76872JF525
Janelia Fluor 549
NBP1-76872JF549
Janelia Fluor 585
NBP1-76872JF585
Janelia Fluor 635
NBP1-76872JF635
Janelia Fluor 646
NBP1-76872JF646
Janelia Fluor 669
NBP1-76872JF669
PerCP
NBP1-76872PCP
mFluor Violet 450 SE
NBP1-76872MFV450
mFluor Violet 500 SE
NBP1-76872MFV500
mFluor Violet 610 SE
NBP1-76872MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-76872-0.025mg
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CX3CR1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids in a extracellular loop of human CX3CR1. The immunogen is located within amino acids 160 - 210 of CX3CR1. Amino Acid Squence: CLGDYPEVLQEIWP
Specificity
At least two isoforms of CX3CR1 are known to exist.
Predicted Species
Mouse (93%), Rat (100%), Rabbit (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CX3CR1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5 ug/ml
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CX3CR1 Peptide (NBP1-76872PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for CX3CR1 Antibody - BSA Free

  • Beta chemokine receptor-like 1
  • CCRL1
  • chemokine (C-C) receptor-like 1
  • chemokine (C-X3-C motif) receptor 1
  • chemokine (C-X3-C) receptor 1
  • CMKbRL1
  • CMK-BRL1
  • CMK-BRL-1
  • CMKDR1
  • CX3C chemokine receptor 1
  • CX3CR1
  • Fractalkine receptor
  • G protein-coupled receptor 13
  • GPR13G-protein coupled receptor 13
  • GPRV28
  • V28
  • V28C-X3-C CKR-1

Background

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-76872
CX3CR1 Antibody - BSA Free
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, ICC/IF, IHC

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CX3CR1 Antibody (NBP1-76872). (Showing 1 - 1 of 1 FAQ).

  1. I'm interested in antibodies against CX3CR1 to use them for IHC-Paraffin protocol in humans. I choose Your product with catalog number NBP1-76872 (0.1 mg). My question is about additional reagents, I maybe need them to perform the staining. Could you tell me whether control probe is needed? Could I receive appropriate protocol with aforesaid antibodies using?
    • Our lab did not use any customized protocol for validation of NBP1-76872 and our general IHC-P protocol may be found at following web-link: Immunohistochemistry-Paraffin Protocol. Because this target is a membrane protein, we would recommend to not to over-fix tissues (approximately 12 hours fixation should be fine and should not exceed 24 hours). Antigen retrieval may be required for this target. It is expressed in lymphoid as well as neural tissues. Spleen and Tonsil tissue would be a nice positive control. We have CX3CR1 Peptide (NBP1-76872PEP) also that you may use for including a blocking negative control in your staining for proving specificity of the antibody.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CX3CR1
Uniprot