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CX3CR1 Antibody [Allophycocyanin]

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Mouse splenocytes were stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A) and Anti-Mouse Integrin aX/CD11c PE-conjugated Monoclonal Antibody . Quadrant markers were set based on control antibody staining (Catalog # IC108A). View our protocol for Staining Membrane-associated Proteins.
Mouse splenocytes were stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A) and Anti-Mouse Integrin aX/CD11c PE-conjugated Monoclonal Antibody . Quadrant ...read more
CHO Chinese hamster ovary cell line transfected with either (A) mouse CX3CR1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 488
FAB5825G-100
Alexa Fluor 488
FAB5825G-025
Allophycocyanin
FAB5825A-100
Phycoerythrin
FAB5825P-025
Phycoerythrin
FAB5825P-100
Unconjugated
AF5825
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF5825
Carrier Free
AF5825
Carrier Free
FAB5825P-025
Carrier Free
FAB5825P-100
Carrier Free
FAB5825A-100
Carrier Free
FAB5825G-025
Carrier Free
FAB5825G-100
Carrier Free
AF5825-SP
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CX3CR1 Antibody [Allophycocyanin] Summary

Immunogen
E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9
Specificity
Detects mouse CX3CR1 in direct ELISAs.  Stains mouse CX3CR1 transfectants but not irrelevant transfectants in flow cytometry.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CX3CR1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB5825A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CR1 Antibody [Allophycocyanin]

  • Beta chemokine receptor-like 1
  • CCRL1
  • chemokine (C-C) receptor-like 1
  • chemokine (C-X3-C motif) receptor 1
  • chemokine (C-X3-C) receptor 1
  • CMKbRL1
  • CMK-BRL1
  • CMK-BRL-1
  • CMKDR1
  • CX3C chemokine receptor 1
  • CX3CR1
  • Fractalkine receptor
  • G protein-coupled receptor 13
  • GPR13G-protein coupled receptor 13
  • GPRV28
  • V28
  • V28C-X3-C CKR-1

Background

CX3CR1 (CX3C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CX3CR1 Antibody
[Allophycocyanin]
FAB5825A
CX3CR1 Antibody [Allophycocyanin]
Species: Mu
Applications: Flow

Publications for CX3CR1 Antibody (FAB5825A)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(3)
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(3)
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Bioinformatics

Gene Symbol CX3CR1
Uniprot