![Western Blot: Crk [p Tyr221] Antibody [NBP3-23252] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Crk (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk (C-terminal region) NBP3-23251 (lanes 1 & 2) and Crk (Tyr-221) phospho-specific NBP3-23252 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23252_rabbit-crk-p-tyr221-pab-19920231252363.jpg "Western Blot: Crk [p Tyr221] Antibody [NBP3-23252] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Crk (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk (C-terminal region) NBP3-23251 (lanes 1 & 2) and Crk (Tyr-221) phospho-specific NBP3-23252 (lanes 3 & 4).")
| Reactivity | Hu, Mu, Rt, Ch, XpSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Phospho-Crk (Tyr-221) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-221 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, and has 40% homology with the conserved site in CrkL (Tyr-207). The site is not found in Crk I. [UniProt# P46108] |
| Modification | p Tyr221 |
| Specificity | The antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. This reactivity is not observed after akaline phosphatase treatment. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CRK |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Crk Antibody (NBP3-23252)Find related products by research area.
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| Gene Symbol | CRK |
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