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CRISP-2 Antibody

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Western Blot: CRISP-2 Antibody [NBP1-52013] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with EB09850 (0.11ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB09850 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Western Blot: CRISP-2 Antibody [NBP1-52013] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with EB09850 (0.11ug/ml) in Lane A and probed with anti-MYC ...read more

Product Details

Summary
Reactivity Hu, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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CRISP-2 Antibody Summary

Immunogen
Peptide with sequence KCTLQHSDPEDRK corresponding to internal region according to NP_003287.1.
Epitope
KCTLQHSDPEDRK
Specificity
Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRISP2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.05 - 0.1 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
Publications
Read Publication using NBP1-52013.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRISP-2 Antibody

  • Cancer/testis antigen 36
  • CRISP2
  • CRISP-2
  • CRISP-2GAPDL5
  • CT36TSP1
  • cysteine-rich secretory protein 2
  • GAPDL5
  • glyceraldehyde-3-phosphate dehydrogenase-like 5
  • testis specific protein 1 (probe H4-1 p3-1)
  • Testis-specific protein TPX-1
  • TPX1
  • TPX1MGC111136
  • TSP1

Background

CRISP2, or Cysteine-rich secretory protein 2, consists of a 243 amino acid isoform that is 27 kDa, and is involved in the regulation of calcium fluxation and ion channel activity during sperm capacitation. Current research is being conducted on CRISP2 and its relation to several diseases and disorders, including male infertility, atopic dermatitis, and sinusitis. The protein is not currently linked to any specific pathways or biological processes, but it interacts with PPP2R1A and PPP2R1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CRISP-2 Antibody
NBP1-52013
CRISP-2 Antibody
Species: Hu, Po, Bv, Ca
Applications: WB, ELISA

Publications for CRISP-2 Antibody (NBP1-52013)(1)


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Bioinformatics

Gene Symbol CRISP2
Entrez
Uniprot