Calreticulin Antibody - BSA Free

Calreticulin, also called CALR, is an endoplasmic reticulum (ER) protein that functions as a calcium (Ca2+) buffering molecular chaperone (1-3). Together with calnexin and ERp57, calreticulin has a critical role in protein folding and quality control of newly synthesized glycoproteins (1-3). Calreticulin has many diverse functions beyond Ca2+ binding, including cell adhesion, lectin binding, apoptosis, nuclear transport, antigen presentation, and immune response (1-3). Specifically, calreticulin is part of the peptide-loading complex that resides on the ER membrane and is responsible for antigen loading onto MHC class I molecules (1-3). Calreticulin protein has a theoretical molecular weight of 46 kDa and is 417 amino acids (aa) in length (1-3). The protein has three main domains: the N-terminal lectin-like domain, the proline-rich P-domain, and the acidic C-domain which is followed by an ER-retention KDEL signal sequence (1-3).

Given its role in multiple biological processes, it makes sense that calreticulin is implicated in both healthy and disease states. Studies have found that calreticulin mutations were present in patients with myeloproliferative neoplasms (MFN) and essential thrombocythaemia (ET) (4). Calreticulin expression is typically upregulated in most cancer lines, however it is downregulated in some tissues including cervical carcinomas, prostate cancer, and human colon adenocarcinoma (3). High expression of calreticulin on cancer cells is related to tumor cell phagocytosis and is often correlated with, and counteracted by, elevated CD47 expression to prevent cancer cell phagocytosis (3). Calreticulin mutations can serve as a major diagnostic biomarker for MFN and ET and additionally the calreticulin gene may be a potential target for cancer therapeutics (3,4).

References

1. Michalak, M., Groenendyk, J., Szabo, E., Gold, L. I., & Opas, M. (2009). Calreticulin, a multi-process calcium-buffering chaperone of the endoplasmic reticulum. The Biochemical Journal. https://doi.org/10.1042/BJ20081847

2. Fucikova, J., Spisek, R., Kroemer, G., & Galluzzi, L. (2021). Calreticulin and cancer. Cell Research. https://doi.org/10.1038/s41422-020-0383-9

3. Sun, J., Mu, H., Dai, K., & Yi, L. (2017). Calreticulin: a potential anti-cancer therapeutic target. Die Pharmazie. https://doi.org/10.1691/ph.2017.7031

4. Prins, D., Gonzalez Arias, C., Klampfl, T., Grinfeld, J., & Green, A. R. (2020). Mutant Calreticulin in the Myeloproliferative Neoplasms. HemaSphere. https://doi.org/10.1097/HS9.0000000000000333

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Hamster, Primate.

We have publications tested in 9 applications: B/N, Cytometric Bead Assay Standard, EM, FLOW, ICC/IF, IF/IHC, IP, PA, WB.

Showing Publications 1 - 10 of 28. Show All 28 Publications. Collapse Publications.

Publications using NB600-101	Applications	Species
Sanchez-Herrero E, Campos-Silva C, Caceres-Martell Y et al. ALK-Fusion Transcripts Can Be Detected in Extracellular Vesicles (EVs) from Nonsmall Cell Lung Cancer Cell Lines and Patient Plasma: Toward EV-Based Noninvasive Testing Clinical chemistry [PMID: 35348673] (ICC/IF, Human)	ICC/IF	Human
Zheng C, Sui B, Zhang X et al. Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes Journal of Extracellular Vesicles 2021-05-01 [PMID: 34084287] (Human)		Human
Harada K, Matsuoka H, Toyohira Y et al. Mechanisms for establishment of GABA signaling in adrenal medullary chromaffin cells Journal of neurochemistry 2021-03-11 [PMID: 33704788]
Roberti MP, Yonekura S, Duong CPM et al. Chemotherapy-induced ileal crypt apoptosis and the ileal microbiome shape immunosurveillance and prognosis of proximal colon cancer Nat. Med. 2020-05-25 [PMID: 32451498] (Mouse)		Mouse
Musante L, Bontha Sv, La Salvia S et Al. Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs Sci Rep Feb 28 2020 [PMID: 32111925] (WB, Human)	WB	Human
Lekszas C, Foresti O, Raote I et Al. Biallelic TANGO1 mutations cause a novel syndromal disease due to hampered cellular collagen secretion Elife 2020-02-26 [PMID: 32101163] (ICC/IF, Human)	ICC/IF	Human
Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human) Details: Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).		Human
Dong X, Wu W, Ma L et al. Collectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production J Innate Immun 2017-08-04 [PMID: 28772263] (FLOW, Mouse)	FLOW	Mouse
Yamada HY, Kumar G, Zhang Y et al. Systemic chromosome instability in Shugoshin-1 mice resulted in compromised glutathione pathway, activation of Wnt signaling and defects in immune system in the lung. Oncogenesis. 2016-08-16 [PMID: 27526110] (WB, IF/IHC)	WB, IF/IHC
Teng CY, van Oers MM, Wu TY. Additive effect of calreticulin and translation initiation factor eIF4E on secreted protein production in the baculovirus expression system. Appl Microbiol Biotechnol. 2013-10-01 [PMID: 23900798] (WB)	WB
Teng CY, Chang SL, Tsai MF, Wu TY. alpha-Synuclein and beta-synuclein enhance secretion protein production in baculovirus expression vector system Appl Microbiol Biotechnol 2013-01-12 [PMID: 23314197] (WB)	WB
Teng CY, Chang SL, van Oers MM, Wu TY. Enhanced Protein Secretion From Insect Cells by Co-Expression of the Chaperone Calreticulin and Translation Initiation Factor eIF4E. Mol Biotechnol. 2012-05-04 [PMID: 22555850] (WB)	WB
Bazwinsky-Wutschke I, Wolgast S, Muhlbauer E, Peschke E. Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma beta-cells (INS-1). Histochem Cell Biol. 2010-07-07 [PMID: 20607274] (Rat)		Rat
erson DJ, Vargas JD, Hsiao JP et al. Recruitment of functionally distinct membrane proteins to chromatin mediates nuclear envelope formation in vivo. J Cell Biol;186(2):183-191. 2009-01-01 [PMID: 19620630] (WB, ICC/IF)	WB, ICC/IF
Baruah P et al. C1q enhances IFN-gamma production by antigen-specific T cells via the CD40 costimulatory pathway on dendritic cells. Blood113(15):3485-93. 2009-04-09 [PMID: 19171874] (FLOW, ICC/IF, Mouse)	FLOW, ICC/IF	Mouse
Jain, P et al. Identification of Human T Cell Leukemia Virus Type 1 Tax Amino Acid Signals Cellular Factors Involved in Secretion of the Viral Oncoprotein. J Biol Chem; 282:34581-34593. 2007-11-01 [PMID: 17897946] (PA, Cytometric Bead Assay Standard, Hamster)	PA, Cytometric Bead Assay Standard	Hamster
Zhou M, He HJ, Suzuki R et al. Localization of sulfonylurea receptor subunits, SUR2A and SUR2B, in rat heart. J Histochem Cytochem 2007-08-01 [PMID: 17438353] (Rat)		Rat
Nurden P et al. Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia. Blood108(8):2587-95. 2006-10-15 [PMID: 16720832] (WB, Human)	WB	Human
Zhou M et al. ATP-sensitive K+-channel subunits on the mitochondria and endoplasmic reticulum of rat cardiomyocytes. J Histochem Cytochem53(12):1491-500. 2005-12-01 [PMID: 15983113] (IF/IHC, WB, Rat)	IF/IHC, WB	Rat
Tsuzaka K, Setoyama Y, Yoshimoto K et al. A splice variant of the TCR zeta mRNA lacking exon 7 leads to the down-regulation of TCR zeta, the TCR/CD3 complex, and IL-2 production in systemic lupus erythematosus T cells. J Immunol174(6):3518-25. 2005-03-15 [PMID: 15749888] (ICC/IF, Human)	ICC/IF	Human
Olesen SH et al. Human FK506 binding protein 65 is associated with colorectal cancer. Mol Cell Proteomics4(4):534-44. 2005-04-01 [PMID: 15671042] (ICC/IF, WB, IF/IHC, Human)	ICC/IF, WB, IF/IHC	Human
Khanna R, Lee EJ, Papazian DM. Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER. J Cell Sci117(Pt 14):2897-908. 2004-06-15 [PMID: 15161937] (IP, Human)	IP	Human
Knox CD et al. Novel role of phospholipase C-delta1: regulation of liver mitochondrial Ca2+ uptake. Am J Physiol Gastrointest Liver Physiol287(3):G533-40. 2004-09-01 [PMID: 15107298] (WB, Rat)	WB	Rat
Somogyi E et al. Calreticulin--an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix. Matrix Biol22(2):179-91. 2003-04-01 [PMID: 12782144] (EM, Rat)	EM	Rat
Ho HC, Suarez SS. Characterization of the intracellular calcium store at the base of the sperm flagellum that regulates hyperactivated motility. Biol Reprod68(5):1590-6. 2003-05-01 [PMID: 12606347] (ICC/IF, Bovine)	ICC/IF	Bovine
Luo X et al. C1q-calreticulin induced oxidative neurotoxicity: relevance for the neuropathogenesis of Alzheimer's disease. J Neuroimmunol135(1-2):62-71. 2003-02-01 [PMID: 12576225] (B/N, ICC/IF, Rat)	B/N, ICC/IF	Rat
Placantonakis D, Cicirata F, Welsh JP. A dominant negative mutation of neuronal connexin 36 that blocks intercellular permeability. Brain Res Mol Brain Res98(1-2):15-28. 2002-01-31 [PMID: 11834292] (ICC/IF, Primate)	ICC/IF	Primate
Woods AJ et al. Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells. J Biol Chem277(8):6428-37. 2002-02-22 [PMID: 11704675] (ICC/IF, WB, Mouse)	ICC/IF, WB	Mouse
Show All 28 Publications. Collapse Publications.

Array NB600-101

• Calreticulin Antibodies
• Calreticulin ELISA Kits
• Calreticulin Lysate
• Calreticulin Protein