Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal ...read more
Contactin-2/TAG1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1714) at 15 ...read more
Simple Western lane view shows lysates of human brain (cerebellum and hippocampus), loaded at 0.2 mg/mL. A specific band was detected for Contactin‑2/TAG1 at approximately 160 kDa (as indicated) using 20 µg/mL ...read more
Mouse myeloma cell line NS0-derived recombinant human Contactin‑2/TAG1 Leu29-Asn1012 Accession # Q02246
Specificity
Detects human Contactin‑2/TAG1 in direct ELISAs. Detects human, mouse, and rat Contactin‑2/TAG1 in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Contactin-3, rhContactin-4, rhContactin-5 and rhContactin-6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CNTN2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Contactin-2/TAG1 Antibody [Unconjugated]
Axonal glycoprotein TAG-1
axonin-1 cell adhesion molecule
Axonin-1
AXT
CNTN2
contactin 2 (axonal)
contactin 2 (transiently expressed)
Contactin2
Contactin-2
DKFZp781D102
FLJ37193
MGC157722
TAG1
TAG-1
TAX
TAX1
TAX-1
TAX1FLJ42746
Transient axonal glycoprotein 1
transiently-expressed axonal glycoprotein
Background
Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl‑phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1‑3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III‑like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3‑5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3‑6). In mature myelinated fibers, Contactin‑2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin‑associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7‑10). The axonal Contactin‑2 interacts in cis with caspr2, and in trans with another Contactin‑2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).
Wolfer, D. & R. J. Giger (1994) Swissprot Accession # Q61330.
Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
Karagogeos, D. (2003) Front. Biosci.8:s1304.
Liu, Y. & M.C. Halloran (2005) J. Neurosci. 25:10556.
Denaxa, M. et al. (2005) Dev. Biol. 288:87.
Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
Traka, M. et al. (2002) J. Neurosci. 22:3016.
Poliak, S. and E. Peles (2003) Nature Reviews Neurosci. 4:968.
Traka, M. et al. (2003) J. Cell Biol. 162:1161.
Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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