I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?

Name: Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-82142
Price: 699 USD

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We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	RtSpecies Glossary
Applications	ELISA
Suitable Sample Type	Serum, Plasma, Cell supernatant
Standard Curve Range	1.56 - 100 ng/mL
Sensitivity	0.94 ng/mL

Specificity	This kit recognizes Rat COMP/Thrombospondin-5 in samples. No significant cross-reactivity or interference between Rat COMP/Thrombospondin-5 and analogues was observed.
Standard Curve Range	1.56 - 100 ng/mL
Sensitivity	0.94 ng/mL
Assay Type	Sandwich-ELISA
Inter-Assay	CV% < 5%
Intra-Assay	CV% < 5%
Spike Recovery	average = 101% for serum; average = 99% for EDTA plasma; average = 93% for cell culture media
Sample Volume	100 uL
Kit Type	ELISA Kit (Colorimetric)
Gene	COMP

Rat COMP/Thrombospondin-5 ELISA Kit (Colorimetric)

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