Home » IBSP/Sialoprotein II » IBSP/Sialoprotein II Proteins » Recombinant Human IBSP/Sialoprotein II Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human IBSP/Sialoprotein II Protein, CF

 (6 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Human IBSP/Sialoprotein II Protein (Catalog # 4014-SP) supports the adhesion of MCF‑7 human breast cancer cells. The ED50 for this effect is 0.0600-0.600 µg/mL.
Recombinant Human IBSP/Sialoprotein II Protein (Catalog # 4014-SP) supports the adhesion of MCF‑7 human breast cancer cells. The ED50 for this effect is 0.0600-0.600 µg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human IBSP/Sialoprotein II Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 of this effect is 0.0600 - 0.600 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived human IBSP/Sialoprotein II protein
Phe17-Gln317, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Phe17
Protein/Peptide Type
Recombinant Proteins
Gene
IBSP
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
34.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-90 kDa, reducing conditions
Publications
Read Publications using
4014-SP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IBSP/Sialoprotein II Protein, CF

  • BNSP
  • Bone sialoprotein 2
  • Bone sialoprotein
  • BSP 2
  • BSP II
  • BSP
  • BSP2
  • BSPII
  • BSP-II
  • Cell binding sialoprotein
  • IBSP
  • Integrin binding sialoprotein
  • SP II
  • SPII
  • SP-II

Background

IBSP (integrin-binding sialoprotein; also BSP or bone sialoprotein (II)) is a 55 - 75 kDa, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins (1 - 3). It is principally associated with the early stages of bone mineralization. BSP is synthesized as a 317 amino acid (aa) precursor that contains a 16 aa signal sequence and a 301 aa mature region (4 - 6). The mature segment is divided into a basic N-terminus (aa 17 - 62), a central region (aa 63 - 233), and an acidic C-terminus (aa 234 - 317) (7).

Functional segments associated with the mature molecule include a type I collagen binding domain (aa 19 - 46), two non-RGD cell binding sites (aa 30 - 57 and 261 - 281), an RGD alpha v beta 3 integrin-binding site (aa 286 - 288) and two potential hydroxyapatite (HAp) nucleation domains (aa 76 - 83 and 151 - 158) (3, 4, 8 - 11). HAp formation requires a BSP nucleation site composed of at least eight consecutive glutamic acid residues and, likely, a contribution from a BSP-associated co-nucleator (10, 12). BSP is highly glycosylated, sulfated, and phosphorylated. Phosphorylation may impact HAp growth, while carbohydrate may regulate cell adhesion (1, 3, 13). Mature human BSP is 70%, 72%, 78%, and 72% aa identical to porcine, rat, canine, and mouse BSP, respectively. BSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells (14 - 17).

  1. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
  2. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
  3. Ganss, B. et al. (1999) Crit. Rev. Oral Biol. Med. 10:79.
  4. Fisher, L.W. et al. (1990) J. Biol. Chem. 265:2347.
  5. Kerr, J.M. et al. (1993) Genomics 17:408.
  6. Kim, R.H. et al. (1994) Matrix Biol. 14:31.
  7. Zaia, J. et al. (2001) Biochemistry 40:12983.
  8. Tye, C.E. et al. (2005) J. Biol. Chem. 280:13487.
  9. Stubbs, J.T. et al. (1997) J. Bone Miner. Res. 12:1210.
  10. Tye, C.E. et al. (2003) J. Biol. Chem. 278:7949.
  11. Miyauchi, A. et al. (1991) J. Biol. Chem. 266:20369.
  12. Hakki, S.S. et al. (2006) J. Periodontol. 77:167.
  13. Wazen, R.M. et al. (2007) J. Histochem. Cytochem. 55:35.
  14. Kacena, M.A. et al. (2006) Bone 39:978.
  15. Bianco, P. et al. (1991) Calcif. Tissue Int. 49:421.
  16. Chen, J. et al. (1992) J. Bone Miner. Res. 7:987.
  17. Kreke, M.R. et al. (2005) Bone 36:1047.

Customers Who Viewed This Item Also Viewed...

Osteopontin/OPN
Antibody [Unconjugate
AF808
Immunocytochemistry Osteopontin/OPN Antibody [Unconjugated]
Neutralization Osteopontin/OPN Antibody [Unconjugated]
Immunohistochemistry Osteopontin/OPN Antibody [Unconjugated]
(2 Reviews)
(208 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
Osteocalcin
Antibody (190125)
MAB1419
Intracellular Staining by Flow Cytometry Osteocalcin Antibody (190125)
Intracellular Staining by Flow Cytometry Osteocalcin Antibody (190125)
Immunocytochemistry Osteocalcin Antibody (190125)
(3 Reviews)
(56 Publications)
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
CXXC1 Antibody
(SR2392)
NBP3-27690
Immunocytochemistry/ Immunofluorescence CXXC1 Antibody (SR2392)
Immunohistochemistry-Paraffin CXXC1 Antibody (SR2392)
Western Blot CXXC1 Antibody (SR2392)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IP, WB
RUNX2/CBFA1
Antibody (232902) [Un ...
MAB2006
Immunocytochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]
Immunohistochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]
(1 Review)
(28 Publications)
Species: Hu
Applications: ICC
Alkaline
Phosphatase/ALPP Anti ...
NB110-3638
Immunocytochemistry/ Immunofluorescence Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
Immunohistochemistry-Paraffin Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
Western Blot Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
(1 Review)
(15 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
ATRNL1 Antibody -
Azide and BSA Free
NBP2-92749
Western Blot ATRNL1 Antibody - Azide and BSA Free
Species: Hu, Mu
Applications: WB
SLPI [HRP]
DPI00
N/A SLPI [HRP]
N/A SLPI [HRP]
(37 Publications)
Species: Hu
Applications: ELISA
CCL27/CTACK [HRP]
DCC270
N/A CCL27/CTACK [HRP]
N/A CCL27/CTACK [HRP]
(10 Publications)
Species: Hu
Applications: ELISA
ASRGL1 Antibody
NBP1-89133
Immunocytochemistry/ Immunofluorescence ASRGL1 Antibody
Immunohistochemistry-Paraffin ASRGL1 Antibody
Immunohistochemistry-Paraffin ASRGL1 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NAT10 Antibody
NBP2-32263
Immunoprecipitation NAT10 Antibody
Western Blot NAT10 Antibody
Immunoprecipitation NAT10 Antibody
Species: Hu, Mu
Applications: IHC, IP, WB
PDLIM3 Antibody
(HL2075) - Azide and ...
NBP3-25358
Immunohistochemistry-Paraffin PDLIM3 Antibody (HL2075) - Azide and BSA Free
Immunohistochemistry-Paraffin PDLIM3 Antibody (HL2075) - Azide and BSA Free
Western Blot PDLIM3 Antibody (HL2075) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
DSPP Antibody -
Azide and BSA Free
NBP2-92546
Western Blot DSPP Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence DSPP Antibody - Azide and BSA Free
(3 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, WB
SPARC Antibody
[Unconjugated]
AF942
Western Blot SPARC Antibody [Unconjugated]
Immunohistochemistry SPARC Antibody [Unconjugated]
Immunocytochemistry SPARC Antibody [Unconjugated]
(38 Publications)
Species: Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow, WB
DMP-1 Antibody
[Unconjugated]
AF4386
Immunohistochemistry DMP-1 Antibody [Unconjugated]
Immunohistochemistry DMP-1 Antibody [Unconjugated]
(3 Publications)
Species: Mu
Applications: IHC, WB
BMP-2
[Unconjugated]
355-BM
Bioactivity BMP-2 [Unconjugated]
(190 Publications)
Species: Hu, Mu, Rt
Applications: BA
Osterix/Sp7
Antibody (764704)
MAB7547
Western Blot Osterix/Sp7 Antibody (764704)
Immunocytochemistry Osterix/Sp7 Antibody (764704)
(1 Review)
(5 Publications)
Species: Hu
Applications: ICC, WB
DMTF1 Antibody
NBP1-84073
Immunohistochemistry-Paraffin DMTF1 Antibody
Immunohistochemistry-Paraffin DMTF1 Antibody
Immunohistochemistry-Paraffin DMTF1 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(555 Publications)
Species: Hu
Applications: BA
Fibronectin
Antibody - BSA Free
NBP1-91258
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
(11 Reviews)
(56 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Recombinant Human
IBSP/Sialoprotein II ...
4014-SP
Recombinant Human IBSP/Sialoprotein II Protein, CF
Species: Hu
Applications: Bioactivity

Publications for IBSP/Sialoprotein II (4014-SP)(6)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 3 applications: Bioassay, ELISA Capture, Western Blot.


Filter By Application
Bioassay
(5)
ELISA Capture
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(4)
N/A
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 4014-SP Applications Species
Thuong, LHH;Huang, CL;Fong, YC;Liu, CL;Guo, JH;Wu, CY;Liu, PI;Tang, CH; Bone sialoprotein facilitates anoikis resistance in lung cancer by inhibiting miR-150-5p expression Journal of cellular and molecular medicine 2024-10-01 [PMID: 39466654] (Bioassay, Human) Bioassay Human
A Umemoto, T Kuwada, K Murata, M Shiokawa, S Ota, Y Murotani, A Itamoto, K Nishitani, H Yoshitomi, T Fujii, A Onishi, H Onizawa, K Murakami, M Tanaka, H Ito, H Seno, A Morinobu, S Matsuda Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis Arthritis Research & Therapy, 2023-02-17;25(1):25. 2023-02-17 [PMID: 36804906] (Bioassay, ELISA Capture, Human, N/A) Bioassay, ELISA Capture Human, N/A
Y Ghochani, SD Muthukrish, A Sohrabi, R Kawaguchi, MC Condro, S Bastola, F Gao, Y Qin, J Mottahedeh, ML Iruela-Ari, N Rao, DR Laks, LM Liau, GW Mathern, SA Goldman, ST Carmichael, I Nakano, G Coppola, SK Seidlits, HI Kornblum A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration Cell Reports, 2022-10-18;41(3):111511. 2022-10-18 [PMID: 36261010] (Bioassay, Human) Bioassay Human
KA White, VJ Cali, RM Olabisi Micropatterning biomineralization with immobilized mother of pearl proteins Scientific Reports, 2021-01-25;11(1):2141. 2021-01-25 [PMID: 33495508] (Bioassay, Human) Bioassay Human
Barros N, Hoac B, Neves R, Addison W, Assis D, Murshed M, Carmona A, McKee M Proteolytic processing of osteopontin by PHEX and accumulation of osteopontin fragments in Hyp mouse bone, the murine model of X-linked hypophosphatemia. J Bone Miner Res, 2013-03-01;28(3):688-99. 2013-03-01 [PMID: 22991293] (Bioassay) Bioassay
Akter R, Rivas D, Geneau G, Drissi H, Duque G Effect of Lamin A/C Knockdown on Osteoblast Differentiation and Function. J. Bone Miner. Res., 2009-02-01;0(0):. 2009-02-01 [PMID: 18847334] (Western Blot) Western Blot

Reviews for IBSP/Sialoprotein II (4014-SP) (0)

There are no reviews for IBSP/Sialoprotein II (4014-SP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IBSP/Sialoprotein II (4014-SP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IBSP/Sialoprotein II Products

Blogs on IBSP/Sialoprotein II

There are no specific blogs for IBSP/Sialoprotein II, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IBSP/Sialoprotein II Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol IBSP
Uniprot