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COMP/Thrombospondin-5 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-99528AF350
Alexa Fluor 405
NBP2-99528AF405
Alexa Fluor 488
NBP2-99528AF488
Alexa Fluor 532
NBP2-99528AF532
Alexa Fluor 594
NBP2-99528AF594
Alexa Fluor 647
NBP2-99528AF647
Alexa Fluor 700
NBP2-99528AF700
Alexa Fluor 750
NBP2-99528AF750
Allophycocyanin
NBP2-99528APC
Biotin
NBP2-99528B
DyLight 350
NBP2-99528UV
DyLight 405
NBP2-99528V
DyLight 488
NBP2-99528G
DyLight 550
NBP2-99528R
DyLight 594
NBP2-99528DL594
DyLight 650
NBP2-99528C
DyLight 680
NBP2-99528FR
DyLight 755
NBP2-99528IR
FITC
NBP2-99528F
HRP
NBP2-99528H
Janelia Fluor 525
NBP2-99528JF525
Janelia Fluor 549
NBP2-99528JF549
Janelia Fluor 585
NBP2-99528JF585
Janelia Fluor 635
NBP2-99528JF635
Janelia Fluor 646
NBP2-99528JF646
Janelia Fluor 669
NBP2-99528JF669
PE
NBP2-99528PE
PerCP
NBP2-99528PCP
Unconjugated
NBP2-99528-100ul
mFluor Violet 450 SE
NBP2-99528MFV450
mFluor Violet 500 SE
NBP2-99528MFV500
mFluor Violet 610 SE
NBP2-99528MFV610
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COMP/Thrombospondin-5 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human COMP/Thrombospondin-5
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COMP
Purity
Antigen and protein A Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen and protein A Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for COMP/Thrombospondin-5 Antibody [CoraFluor™ 1]

  • cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
  • cartilage oligomeric matrix protein
  • cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
  • COMP
  • EDM1
  • EPD1
  • MED
  • MEDMGC131819
  • MGC149768
  • PSACH
  • pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
  • THBS5
  • Thrombospondin5
  • Thrombospondin-5
  • TSP5

Background

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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COMP/Thrombospondin-5 ...
NBP2-99528CL1
COMP/Thrombospondin-5 Antibody [CoraFluor™ 1]
Species: Mu
Applications: WB

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for COMP/Thrombospondin-5 Antibody (NBP2-99528CL1). (Showing 1 - 1 of 1 FAQ).

  1. I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
    • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COMP