What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.

I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NB600-450, the dilution range is 1:1000-1:2000.

We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

How long does delivery take for your collagen 1 alpha 1 antibody?

Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

Collagen I alpha 1 Antibody (COL-1) (NB600-450): Novus Biologicals

Name: Collagen I alpha 1 Antibody (COL-1)
Brand: Novus Biologicals
SKU: NB600-450
Rating: 4.6 (5 reviews)

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Publications using NB600-450	Applications	Species
You Min Kim, Min-Soo Ghim, Meiling Quan, Young Yul Kim, Young-Sam Cho, Alberto Romero García Experimental Verification of the Impact of the Contact Area between the Defect Site and the Scaffold on Bone Regeneration Efficacy Polymers 2024-01-26 [PMID: 38337228]
Zahra Shabani, Joana Schuerger, Xiaonan Zhu, Chaoliang Tang, Li Ma, Alka Yadav, Rich Liang, Kelly Press, Shantel Weinsheimer, Annika Schmidt, Calvin Wang, Abinav Sekhar, Jeffrey Nelson, Helen Kim, Hua Su Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse. Cells 2024-01-12 [PMID: 38201296]
Yusuke Shigeta, Tarek Saleh, Giada Benedetti, Lorenzo Caciolli, Jinke Chang, Elisa Zambaiti, Lei Wu, Sahira Khalaf, Wulei Song, Alessandro Filippo Pellegata, Giovanni Giuseppe Giobbe, Paolo De Coppi Stomach engineering: region-specific characterization of the decellularized porcine stomach. Pediatric surgery international 2023-12-01 [PMID: 38032517]
Lu G, Li X, Wang P et al. Polysaccharide-Based Composite Hydrogel with Hierarchical Microstructure for Enhanced Vascularization and Skull Regeneration Biomacromolecules 2023-09-20 [PMID: 37729544]
Ha H, Lee CH, Lee KS et al. Shape-Configurable Mesh for Hernia Repair by Synchronizing Anisotropic Body Motion Small (Weinheim an der Bergstrasse, Germany) 2023-07-25 [PMID: 37490554] (IHC, Rat)	IHC	Rat
Simon KS, Coelho LC, Veloso PHH et al. Innovative Pre-Clinical Data Using Peptides to Intervene in the Evolution of Pulmonary Fibrosis International journal of molecular sciences 2023-07-04 [PMID: 37446227] (IHC-P, Mouse)	IHC-P	Mouse
Ross Eric Beaumont, Emily Josephine Smith, Lexin Zhou, Neil Marr, Chavaunne T. Thorpe, Deborah Jane Guest Exogenous interleukin-1 beta stimulation regulates equine tenocyte function and gene expression in three-dimensional culture which can be rescued by pharmacological inhibition of interleukin 1 receptor, but not nuclear factor kappa B, signaling Molecular and Cellular Biochemistry 2023-06-14 [PMID: 37314623]
Hazari Y, Urra H, Garcia Lopez VA et al. The endoplasmic reticulum stress sensor IRE1 regulates collagen secretion through the enforcement of the proteostasis factor P4HB/PDIA1 contributing to liver damage and fibrosis bioRxiv : the preprint server for biology 2023-05-02 [PMID: 37205565] (WB, Mouse)	WB	Mouse
Li H, Zhu X, Cao X et Al. Single-cell analysis reveals lysyl oxidase (Lox)(+) fibroblast subset involved in cardiac fibrosis of diabetic mice J Adv Res 2023-01-24 [PMID: 36706988]
Spiaggia G, Taladriz-Blanco P, Hengsberger S et al. Corticostriatal Projections Relying on GABA Levels Mediate Exercise-Induced Functional Recovery in Cerebral Ischemic Mice Biomedicines 2022-12-22 [PMID: 36672538] (EM, Rat) Details: Appendix Figure A3. cLSM micrograph of collagen type I functionalized PDMS films immunostained with mouse monoclonal IgGG1 used as primary antibody and DyLight 488 employed for antibody labelling and prepared at 2000 rpm	EM	Rat
Ramani K, Mavila N, Abeynayake A et al. Targeting A-kinase anchoring protein 12 phosphorylation in hepatic stellate cells regulates liver injury and fibrosis in mouse models eLife 2022-10-04 [PMID: 36193675] (Immunoprecipitation)	Immunoprecipitation
Rauf S, Qazi H, Luo J et al. Real-time, highly sensitive detection of alpha-fetoprotein in biological fluids using a QCM sensor based on a Cu2O@MoS2-Au nanocomposite and gold staining IEEE Sensors Journal 2021-12-10
Samra T, Gomez-Gomez T, Linowiecka K et al. Melatonin Exerts Prominent, Differential Epidermal and Dermal Anti-Aging Properties in Aged Human Eyelid Skin Ex Vivo International journal of molecular sciences 2023-11-04 [PMID: 37958946] (IHC-Fr, Human) Details: 1:500 dilution	IHC-Fr	Human
Huynh T, Reed C, Blackwell Z et al. Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injury Scientific reports 2023-02-03 [PMID: 36737628] (IHC-Fr, Rat)	IHC-Fr	Rat
Li H, Zhao T, Cao F et al. Integrated bioactive scaffold with aptamer targeted stem cell recruitment and growth factor induced pro differentiation effects for anisotropic meniscal regeneration Bioengineering & Translational Medicine 2022-03-03 [PMID: 36176622] (IHC-P, Rabbit)	IHC-P	Rabbit
H Lena, G David, K Johann, J Yvonne, Z Frank G, H Daniela, B Roman, B Rolf E, R Nicole In situ regeneration of nasal septal defects using acellular cartilage enhanced with platelet-derived growth factor Journal of tissue engineering, 2022-09-20;13(0):2041731422111. 2022-09-20 [PMID: 36158899]
Yang C, Zhu C, Li Y et al. Injectable selenium-containing polymeric hydrogel formulation for effective treatment of myocardial infarction Frontiers in Bioengineering and Biotechnology 2022-07-11 [PMID: 36032710] (Western Blot)	Western Blot
McClellan P, Ina JG, Knapik DM et al. Mesenchymal Stem Cell Delivery via Topographically Tenoinductive Collagen Biotextile Enhances Regeneration of Segmental Tendon Defects The American journal of sports medicine 2022-07-01 [PMID: 35647785]
Gan Z, Zhao Y, Wu Y et al. Three-dimensional, biomimetic electrospun scaffolds reinforced with carbon nanotubes for temporomandibular joint disc regeneration Acta biomaterialia 2022-05-10 [PMID: 35562008] (ICC/IF, Rat)	ICC/IF	Rat
Verma SK, Yaghoobi H, Slaine P Et al. Multi-pin contact drawing enables production of anisotropic collagen fiber substrates for alignment of fibroblasts and monocytes Colloids Surf B Biointerfaces 2022-05-02 [PMID: 35500531] (Rat) Details: Citation using the Janelia Fluor 549 version of this antibody.		Rat
Kim JT, Roberts K, Dunlap G et al. Nandrolone supplementation does not improve functional recovery in an aged animal model of volumetric muscle loss injury Journal of tissue engineering and regenerative medicine 2022-02-03 [PMID: 35113494] (IF/IHC, Rat)	IF/IHC	Rat
Gundogdu G, Okhunov Z, Starek S et al. Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model Frontiers in bioengineering and biotechnology 2021-12-07 [PMID: 34950646] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Struijk C, Van Genechten W, Verdonk P et al. Human meniscus allograft augmentation by allogeneic mesenchymal stromal/stem cell injections Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2021-05-10 [PMID: 33969529]
Wei Q, Kong N, Liu X et al. Pirfenidone attenuates synovial fibrosis and postpones the progression of osteoarthritis by anti-fibrotic and anti-inflammatory properties in vivo and in vitro Journal of translational medicine 2021-04-19 [PMID: 33874948] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L et al. Adult human liver slice cultures: Modelling of liver fibrosis and evaluation of new anti-fibrotic drugs World Journal of Hepatology 2021-02-27 [PMID: 33708350] (Western Blot)	Western Blot
Kuzan A, Wisniewski J, Maksymowicz K et al. Relationship between calcification, atherosclerosis and matrix proteins in the human aorta Folia Histochemica et Cytobiologica 2021-03-31 [PMID: 33560515] (Immunohistochemistry)	Immunohistochemistry
Meng X, Grad S, Wen C et al. An impaired healing model of osteochondral defect in papain-induced arthritis J Orthop Translat 2021-01-13 [PMID: 33437629]
Marvin K, Schwartz I, Utz E et al. Effects of Fractional CO2 Laser Treatment on Subglottic Scar in a Rabbit Model Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 2020-12-08 [PMID: 33287672] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline)	ICC/IF	Feline
Huynh T, Kim J, Dunlap G et al. In-vivo testing of an injectable matrix gel for the treatment of shoulder cuff muscle fatty degeneration Journal of Shoulder and Elbow Surgery 2020-06-01 [PMID: 32713662] (IHC-Fr, IF/IHC, Rat, Rabbit)	IHC-Fr, IF/IHC	Rat, Rabbit
RINCHIUSO A Interaction of an easy-to-use tissue-engineered collagen-based model for in vitro stent evaluation Thesis
Wang Y, Xiao Y, Long S et al. Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation Sci Adv 2020-07-17 [PMID: 32671214]
Limraksasin P, Okawa H, Zhang M et al. Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs Stem Cells International 2020-05-14 [PMID: 32508932] (IHC-P, Mouse)	IHC-P	Mouse
Liu J, Yu C, Chen Y et al. Fast fabrication of stable cartilage-like tissue using collagen hydrogel microsphere culture J Mater Chem B 2020-04-09 [PMID: 32264594]
Liu J, Wang X, Lu G et Al. Bionic cartilage acellular matrix microspheres as a scaffold for engineering cartilage J Mater Chem B 2020-07-22 [PMID: 32254797]
Yu C, Liu J, Lu G et al. Repair of osteochondral defects in a rabbit model with artificial cartilage particulates derived from cultured collagen-chondrocyte microspheres. J Mater Chem B 2020-04-08 [PMID: 32254543]
Zwaans Bmm, Wegner Ka, Bartolone Sn et Al. Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice Physiol Rep 2020-02-01 [PMID: 32109348] (ICC/IF, Mouse)	ICC/IF	Mouse
Rybinska I, Sandri M, Bianchi F et al. Extracellular Matrix Features Discriminate Aggressive HER2-Positive Breast Cancer Patients Who Benefit from Trastuzumab Treatment Cells 2020-02-13 [PMID: 32069815] (IHC-Fr, Human)	IHC-Fr	Human
Limraksasin P, Kondo T, Zhang M et al. In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells Int J Mol Sci 2020-01-16 [PMID: 31963264] (IF/IHC, Mouse)	IF/IHC	Mouse
Willemse J, Verstegen M, Vermeulen A et al. Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion Materials Science and Engineering: C 2019-09-12 [PMID: 31923991] (IF/IHC, IHC-P, Human)	IF/IHC, IHC-P	Human
Robbach BP, Gulecyuz MF, Kempfert L, et al. Rotator Cuff Repair With Autologous Tenocytes and Biodegradable Collagen Scaffold: A Histological and Biomechanical Study in Sheep Am J Sports Med 2019-12-16 [PMID: 31841352]
Chen M, Feng Z, Guo W et al. PCL-MECM-Based Hydrogel Hybrid Scaffolds and Meniscal Fibrochondrocytes Promote Whole Meniscus Regeneration in a Rabbit Meniscectomy Model ACS Appl Mater Interfaces 2019-10-22 [PMID: 31596568]
Jallow F, O'Leary KA, Rugowski DE et al. Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer Oncogene 2019-08-12 [PMID: 31406251]
Ligresti G, Caporarello N, Meridew JA et al. Status of growth plates can be monitored by MRI J Magn Reson Imaging 2019-05-01 [PMID: 31044458] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Wu D, Wang Z, Wang J et al. Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment Stem Cell Res Ther 2018-11-28 [PMID: 30486863] (WB, Rabbit)	WB	Rabbit
Learn G D, McClellan P E et al. Woven collagen biotextiles enable mechanically functional rotator cuff tendon regeneration during repair of segmental tendon defects in vivo. J Biomed Mater Res B Appl Biomater 2019-01-08 [PMID: 30485649] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Costa D, Vene R, Benelli R et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts Front Immunol 2018-05-29 [PMID: 29910806] (Human)		Human
Kuzan A, Chwilkowska A, Maksymowicz K et al. Advanced glycation end products as a source of artifacts in immunoenzymatic methods Glycoconj. J. 2018-01-05 [PMID: 29305778] (ELISA, Human)	ELISA	Human
Ramani K, Tomasi ML, Berlind J et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation Am. J. Pathol. 2018-01-02 [PMID: 29305319] (Human)		Human
Liu G Y, Agarwal R, Ko K R et al. Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D. Scientific Reports 2017-01-01 [PMID: 28852121] (IF/IHC, Mouse)	IF/IHC	Mouse
Wegner K, Keikhosravi A, Eliceiri K et al. Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections. J Histochem Cytochem 2017-07-10 [PMID: 28692327] (IHC-P, Mouse)	IHC-P	Mouse
da Silva IR, Tiveron LCRDC, da Silva MV et al. In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars. Mediators Inflamm. 2017-06-22 [PMID: 28638180] (IF/IHC, Human)	IF/IHC	Human
Du Y, Liu H, Yang Q et al. Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits. Biomaterials. 2017-08-01 [PMID: 28528301] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Bono N, Meghezi S, Soncini M et al. A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models. Ann Biomed Eng. 2017-02-21 [PMID: 28224370]
Sa G, Xiong X, Ren J et al. Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models. Eur J Pharm Sci 2016-10-18 [PMID: 27769912] (ICC/IF, IF/IHC, Human)	ICC/IF, IF/IHC	Human
Karti O, Zengin MO, Cinar E et al. Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations. Cornea. 2016-10-12 [PMID: 27741014]
Loy C, Laine A, Mantovani D. Rotation-based technique for the rapid densification of tubular collagen gel scaffolds. Biotechnol J 2016-12-01 [PMID: 27731544]
Cheng MQ, Wahafu T, Jiang GF et al. A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration. Sci Rep. 2016-04-13 [PMID: 27071777] (IHC-P, Human)	IHC-P	Human
Geburek F, Lietzau M, Beineke A et al. Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies Stem Cell Res Ther 2015-07-24 [PMID: 26113022] (IHC-P, Equine) Details: Collagen I alpha 1 /COL 1A1 antibody was used for IHC-P analysis of longitudinal sections of tendon biopsies from superficial digital flexor tendons with tendinopathy from horses that were subjected or not to single injection of autologous conditioned serum (ACS).	IHC-P	Equine
Otani Y, Komura M, Komura H et al. Optimal amount of basic fibroblast growth factor (b-FGF) in gelatin sponges incorporating B-tricalcium phosphate (TCP) with chondrocytes. Tissue Eng Part A. 2014-10-06 [PMID: 25287675] (IF/IHC, Mouse)	IF/IHC	Mouse
Triulzi T, Ratti M, Tortoreto M et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2013-11-14 [PMID: 24242003] (IHC-P, Human)	IHC-P	Human
You W, Fan L, Duan D et al. Foxc2 over-expression in bone marrow mesenchymal stem cells stimulates osteogenic differentiation and inhibits adipogenic differentiation. Mol Cell Biochem. 2013-10-12 [PMID: 24122419] (ICC/IF, Rabbit)	ICC/IF	Rabbit
Murali S, Rai B, Dombrowski C et al. Affinity-selected heparan sulfate for bone repair. Biomaterials 2013-04-27 [PMID: 23632323] (IHC-P, Rabbit)	IHC-P	Rabbit
Re’em T, Kaminer-Israeli Y, Ruvinov E, Cohen S. Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds. Biomaterials. 2011-10-21 [PMID: 22019120]
Krasinski Z, Biskupski P, Dzieciuchowicz L et al. The influence of elastic components of the venous wall on the biomechanical properties of different veins used for arterial reconstruction. Eur J Vasc Endovasc Surg 2010-08-01 [PMID: 20570188] (IF/IHC, Human)	IF/IHC	Human
Show All 65 Publications. Collapse Publications.

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Details

reviewed by:
Verified Customer

IHC-P

Mouse

06/24/2021

View

Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	Liver
Species	Mouse
Lot	NB600-450

Comments

Primary antibody dilution 1:200
Enzymatic/proteolytic antigen retrieval
No Permeabilization

Enlarge

Flow

Goat

01/22/2019

Application	Flow Cytometry
Sample Tested	Chondrocyte
Species	Goat
Lot	067M4805V

IHC-Fr

Feline

09/15/2018

Application	Immunohistochemistry-Frozen
Sample Tested	Sclera
Species	Feline
Lot	067M4805V

Immunocytochemistry Collagen I alpha 1 NB600-450

ICC

Pig

06/07/2017

Application	Immunocytochemistry
Sample Tested	Pig trabecular meshwork cells
Species	Pig
Lot	N/A

Immunofluorescence Collagen I alpha 1 NB600-450

reviewed by:
Jessica Ochoa

Other

04/21/2015

Application	Immunofluorescence
Sample Tested	Equine Tenocytes
Species	Other

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, Bv, Eq, Fe, Ma, RbSpecies Glossary
Applications	WB, DB, ELISA, ICC/IF, IHC
Clone	COL-1
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Immunogen	This Collagen I alpha 1 antibody (COL-1) was raised against full length bovine native protein (purified).
Epitope	The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding.
Localization	Extracellular matrix
Specificity	This Collagen I alpha 1 antibody (COL-1) recognizes the native (helical) form of collagen type I using ELISA and dot-blot. It does not react with the thermally-denatured molecule, and shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	COL1A1
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Ascites
Preservative	0.09% Sodium Azide
Purity	Unpurified

	Verified Customer 06/24/2021
Application:	IHC-P
Species:	Mouse

Collagen I alpha 1 Antibody (COL-1)

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Publications for Collagen I alpha 1 Antibody (NB600-450)(65)

Reviews for Collagen I alpha 1 Antibody (NB600-450) (5) 4.65

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	Verified Customer 09/15/2018
Application:	IHC-Fr
Species:	Feline

Gene Symbol	COL1A1
Entrez	1277(Human) 29393(Rat)