Collagen III alpha 1/COL3A1 Antibody

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Feline, Mammal, Sheep.

We have publications tested in 10 applications: Block/Neutralize, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, WB, Western Blot.

Showing Publications 1 - 10 of 50. Show All 50 Publications. Collapse Publications.

Publications using NB600-594	Applications	Species
Lam Y, Chan C, Geng L et al. APLNR marks a cardiac progenitor derived with human induced pluripotent stem cells bioRxiv 2023-02-23 (Immunocytochemistry/ Immunofluorescence, Human)	Immunocytochemistry/ Immunofluorescence	Human
Yang G, Xiang J, Yang X et al. Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2 eLife 2024-11-04 [PMID: 39495216]
Osiris Germán Idelfonso-García, Brisa Rodope Alarcón-Sánchez, Dafne Guerrero-Escalera, Norma Arely López-Hernández, José Luis Pérez-Hernández, Ruth Pacheco-Rivera, Jesús Serrano-Luna, Osbaldo Resendis-Antonio, Erick Andrés Muciño-Olmos, Diana Ivette Aparicio-Bautista, Gustavo Basurto-Islas, Rafael Baltiérrez-Hoyos, Verónica Rocío Vásquez-Garzón, Saúl Villa-Treviño, Pablo Muriel, Héctor Serrano, Julio Isael Pérez-Carreón, Jaime Arellanes-Robledo, Marco Fiore Nucleoredoxin Redox Interactions Are Sensitized by Aging and Potentiated by Chronic Alcohol Consumption in the Mouse Liver Antioxidants 2024-02-20 [PMID: 38539791]
Camarena, V;Williams, MM;Morales, AA;Zafeer, MF;Kilic, OV;Kamiar, A;Abad, C;Rasmussen, MA;Briski, LM;Peart, L;Bademci, G;Barbouth, DS;Smithson, S;Wang, G;Shehadeh, LA;Walz, K;Tekin, M; ADAMTSL2 mutations determine the phenotypic severity in Geleophysic Dysplasia JCI insight 2024-02-01 [PMID: 38300707]
Laura Jahnke, Virginie Perrenoud, Souska Zandi, Yuebing Li, Federica Maria Conedera, Volker Enzmann, Girish Kumar Srivastava Modulation of Extracellular Matrix Composition and Chronic Inflammation with Pirfenidone Promotes Scar Reduction in Retinal Wound Repair Cells 2024-01-16 [PMID: 38247855]
Ito A, Ohnuki Y, Suita K et al. Effects of the angiotensin-converting enzyme inhibitor captopril on occlusal-disharmony-induced cardiac dysfunction in mice Scientific reports 2023-11-15 [PMID: 37968296] (WB, Mouse) Details: 1:1000 dilution	WB	Mouse
Paul K, Darzi S, Del Borgo M et al. Vaginal delivery of tissue engineered endometrial mesenchymal stem/stromal cells in an aloe vera-alginate hydrogel alleviates maternal simulated birth injury Applied Materials Today 2021-03-01 (ICC/IF, Human)	ICC/IF	Human
Lam YY, Chan CH, Geng L et al. APLNR marks a cardiac progenitor derived with human induced pluripotent stem cells Heliyon 2023-07-13 [PMID: 37539315] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Ha H, Lee CH, Lee KS et al. Shape-Configurable Mesh for Hernia Repair by Synchronizing Anisotropic Body Motion Small (Weinheim an der Bergstrasse, Germany) 2023-07-25 [PMID: 37490554] (IHC, Rat)	IHC	Rat
Hernández-Bule ML, Toledano-Macías E, Pérez-González LA et al. Anti-Fibrotic Effects of RF Electric Currents International journal of molecular sciences 2023-07-01 [PMID: 37446165] (ICC/IF)	ICC/IF
Antonova L, Kutikhin A, Sevostianova V et al. Controlled and Synchronised Vascular Regeneration upon the Implantation of Iloprost- and Cationic Amphiphilic Drugs-Conjugated Tissue-Engineered Vascular Grafts into the Ovine Carotid Artery: A Proteomics-Empowered Study Polymers (Basel) 2022-11-26 [PMID: 36501545] (Block/Neutralize)	Block/Neutralize
Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis Jul 13 1905
Zhao T, Chu Z, Chu C et al. Macrophages induce gingival destruction via Piezo1-mediated MMPs-degrading collagens in periodontitis Frontiers in Immunology 2023-05-16 [PMID: 37261355] (ICC/IF, Human, Mouse) Details: Dilution: 1:100	ICC/IF	Human, Mouse
Antonova LV, Sevostianova VV, Silnikov VN et al. Comparison of the Patency and Regenerative Potential of Biodegradable Vascular Prostheses of Different Polymer Compositions in an Ovine Model International journal of molecular sciences 2023-05-10 [PMID: 37239889] (IHC-Fr, Sheep)	IHC-Fr	Sheep
Su CY, Liu TY, Wang HV, Yang WC Histopathological Study on Collagen in Full-Thickness Wound Healing in Fraser's Dolphins (Lagenodelphis hosei) Animals : an open access journal from MDPI 2023-05-18 [PMID: 37238111] (IHC-P, Mammal) Details: 1:200 IHC-P dilution. Dolpin is the species	IHC-P	Mammal
Wu X, Zhang D, Qiao X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis The European respiratory journal 2023-06-01 [PMID: 36758986] (Western Blot, Mouse)	Western Blot	Mouse
Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis 1905-07-13
B Wang, Y Tan, Y Zhang, S Zhang, X Duan, Y Jiang, T Li, Q Zhou, X Liu, Z Zhan Loss of KDM5B ameliorates pathological cardiac fibrosis and dysfunction by epigenetically enhancing ATF3 expression Experimental & Molecular Medicine, 2022-12-08;0(0):. 2022-12-08 [PMID: 36481938]
Latif A, Fisher LE, Dundas AA et al. Microparticles Decorated with Cell-Instructive Surface Chemistries Actively Promote Wound Healing Advanced materials (Deerfield Beach, Fla.) 2022-11-28 [PMID: 36440539] (IHC-P, Human)	IHC-P	Human
Wang B, Tan Y, Zhou W et al. Loss of BTK ameliorates the pathological cardiac fibrosis and dysfunction Matrix biology : journal of the International Society for Matrix Biology 2022-08-25 [PMID: 36031013]
Yan S, Peng Y, Lu J et al. Differential Requirement of DICER1 Activity during Development of Mitral and Tricuspid Valves J Cell Sci 2022-08-10 [PMID: 35946425]
Madison J, Wilhelm K, Meehan DT et al. Glomerular basement membrane deposition of collagen ?1(III) in Alport glomeruli by mesangial filopodia injures podocytes via aberrant signaling through DDR1 and integrin ?2?1 The Journal of Pathology 2022-09-01 [PMID: 35607980]
Srinivasan MP, Bhopale KK, Caracheo AA et al. Exposure to binge ethanol and fatty acid ethyl esters exacerbates chronic ethanol-induced pancreatic injury in hepatic alcohol dehydrogenase deficient deer mice American journal of physiology. Gastrointestinal and liver physiology 2022-01-05 [PMID: 34984929] (WB, Mouse)	WB	Mouse
Guillaume E, Zacharopoulou M, Reynolds B et al. Additional Value of Second Harmonic Generation Microscopy in the Diagnosis of Feline Collagen Type III Glomerulopathy Journal of Comparative Pathology 2021-10-01 [PMID: 34686276] (IHC-P, Feline)	IHC-P	Feline
Maity P, Singh K, Krug L Et al. Persistent JunB activation in fibroblasts disrupts stem cell niche interactions enforcing skin aging Cell reports 2021-08-31 [PMID: 34469740] (ICC/IF, Mouse)	ICC/IF	Mouse
Antonova LV, Krivkina EO, Sevostianova VV Et al. Tissue-Engineered Carotid Artery Interposition Grafts Demonstrate High Primary Patency and Promote Vascular Tissue Regeneration in the Ovine Model Polymers 2021-08-08 [PMID: 34451177] (IF/IHC, Sheep)	IF/IHC	Sheep
Oka SI, Byun J, Huang CY, Imai N, Ralda G, Zhai P, Xu X, Kashyap S, Warren JS, Alan Maschek J, Tippetts TS, Tong M, Venkatesh S, Ikeda Y, Mizushima W, Kashihara T, Sadoshima J. Nampt Potentiates Antioxidant Defense in Diabetic Cardiomyopathy 2021-04-30 [PMID: 33928788] (WB, Mouse)	WB	Mouse
Li W, Qi N, Wang S et al. miR-455-5p regulates atrial fibrillation by targeting suppressor of cytokines signaling 3 Journal of physiology and biochemistry 2021-04-01 [PMID: 33792885]
Huang L, Zhang Y, Yang J et al. EGR 1 plays a protective role in AMPK inhibitor compound C induced apoptosis through ROS induced ERK activation in skin cancer cellsAnti fibrotic effects and the mechanism of action of miR 29c in silicosis Molecular medicine reports 2021-04-01 [PMID: 33649785]
Li H, Wang Y, Liu J, et al. Endothelial Klf2-Foxp1-TGF beta signal mediates the inhibitory effects of simvastatin on maladaptive cardiac remodeling Theranostics 2021-01-01 [PMID: 33408770] (WB)	WB
Birchmeier C, Akalin A, Spuler S et al. Single-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cells Nat Commun 2020-12-14 [PMID: 33311457]
Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline)	ICC/IF	Feline
Melchor, S J, Hatter, J A Et al. T. gondii infection induces IL-1R dependent chronic cachexia and perivascular fibrosis in the liver and skeletal muscle. Sci Rep 2020-09-24 [PMID: 32973293] (FLOW, Human)	FLOW	Human
Zwaans Bmm, Wegner Ka, Bartolone Sn et Al. Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice Physiol Rep 2020-02-01 [PMID: 32109348] (ICC/IF, Mouse)	ICC/IF	Mouse
Willemse J, Verstegen M, Vermeulen A et al. Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion Materials Science and Engineering: C 2019-09-12 [PMID: 31923991] (IF/IHC, IHC-P, Human)	IF/IHC, IHC-P	Human
Guo Xiangjiang, Fereydooni Arash, Isaji Toshihiko et al. Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency. Scientific Reports 2019-07-30 [PMID: 31363142] (IHC-P, Mouse)	IHC-P	Mouse
Li G, Chan Y. L, et al. A Mitochondrial Specific Antioxidant Reverses Metabolic Dysfunction and Fatty Liver Induced by Maternal Cigarette Smoke in Mice. Nutrients 2019-07-21 [PMID: 31330878] (IF/IHC, Mouse)	IF/IHC	Mouse
Yang J, Wang B, Li N et al. Salvia miltiorrhiza and Carthamus tinctorius Extract Prevents Cardiac Fibrosis and Dysfunction after Myocardial Infarction by Epigenetically Inhibiting Smad3 Expression Evid Based Complement Alternat Med. 2019-05-30 [PMID: 31275414] (WB, Mouse)	WB	Mouse
Wang X, Pan J, Liu H et al. AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model Life Sci. 2019-02-18 [PMID: 30790610] (IHC-P, WB, Rat)	IHC-P, WB	Rat
Zhang M, Zhu P, Wang Y et al. Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload. J. Cell. Mol. Med. 2018-11-08 [PMID: 30411499] (WB, Rat)	WB	Rat
Tian HP, Sun YH, He L et al. Single-Stranded DNA-Binding Protein 1 Abrogates Cardiac Fibroblast Proliferation and Collagen Expression Induced by Angiotensin II. Int Heart J. 2018-10-25 [PMID: 30369577]
Angel PM, Comte-Walters S, Ball LE et al. Mapping Extracellular Matrix Proteins in Formalin-Fixed, Paraffin-embedded Tissues by MALDI Imaging Mass Spectrometry J. Proteome Res. 2017-11-21 [PMID: 29161047] (IHC-P)	IHC-P
Schreckenberg R, Horn AM, da Costa Rebelo RM et al. Effects of 6-months' Exercise on Cardiac Function, Structure and Metabolism in Female Hypertensive Rats-The Decisive Role of Lysyl Oxidase and Collagen III Front Physiol 2017-08-21 [PMID: 28824452] (WB, Rat) Details: Collagen III antibody was used to look at the effect of running training on the expression of COL III in rats that were either sedentary or subjected to running training	WB	Rat
Teo Z, Chan JSK, Chong HC et al. Angiopoietin-like 4 induces a B-catenin-mediated upregulation of ID3 in fibroblasts to reduce scar collagen expression Sci Rep 2017-07-24 [PMID: 28740178] (WB, Human)	WB	Human
Lee HJ, Kim YS, Ok JH et al. Effect of a single subacromial prednisolone injection in acute rotator cuff tears in a rat model. Knee Surg Sports Traumatol Arthrosc 2013-01-31 [PMID: 23370982] (IF/IHC, Rat)	IF/IHC	Rat
Mak KM, Chu E, Lau KH, Kwong AJ. Liver fibrosis in elderly cadavers: localization of collagen types I, III, and IV, alpha-smooth muscle actin, and elastic fibers. The Anatomical Record, 2012; vol 295, pages 1159-1167. 2012-07-01 [PMID: 22644959]
Mak KM, Kwong AJ, Chu E et al. Hepatic steatosis, fibrosis, and cancer in elderly cadavers. Anat Rec (Hoboken) 2012-01-01 [PMID: 22139908]
Hechler B, Gachet C Comparison of two murine models of thrombosis induced by atherosclerotic plaque injury. Thromb Haemost 2011-05-01 [PMID: 21479341] (IF/IHC, Mouse)	IF/IHC	Mouse
Dahl SL, Kypson AP, Lawson JH et al. Readily available tissue-engineered vascular grafts. Sci Transl Med 2011-02-02 [PMID: 21289273]
Sales VL, Engelmayr GC Jr, Johnson JA Jr et al. Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells Circulation [PMID: 17846326] Details: Used the Biotin form of this antibody.
Show All 50 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Shinichi Oka	WB	Mouse	04/07/2021	View Summary Application Western Blot Sample Tested Heart lysates Species Mouse Lot 42255 Comments Comments Mouse heart lysate was prepared after 3 months of normal diet (ND) and high fat diet (HFD) consumption. Western blot was performed with anti-Col3a1 antibody.
Enlarge	5 reviewed by: Verified Customer	WB	Human	06/07/2018	View Summary Application Western Blot Sample Tested whole cell lysate Species Human Lot 37149
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Feline	02/18/2018	View Summary Application Immunohistochemistry-Frozen Sample Tested Connective tissue Species Feline Lot 39661
Enlarge	5 reviewed by: wang xuyang	IHC-P	Rat	12/20/2017	View Summary Application Immunohistochemistry-Paraffin Sample Tested H9c2 whole cell lysate Species Rat Lot lot37797
Enlarge	4 reviewed by: Verified Customer	IHC-P	Rat	07/20/2016	View Summary Application Immunohistochemistry-Paraffin Sample Tested rat colon Species Rat Lot 33577
Enlarge	5 reviewed by: Jessica Ochoa	IF	Human	04/02/2015	View Summary Application Immunofluorescence Sample Tested HT-1080 (Fibrosarcoma) Species Human
Enlarge	5 reviewed by: Verified Customer	IF	Human	10/04/2014	View Summary Application Immunofluorescence Sample Tested human primary ventricular cardiac fibroblasts (from ScienCell) Species Human Lot 30240

Array NB600-594

• Collagen III alpha 1/COL3A1 Antibodies
• Collagen III alpha 1/COL3A1 ELISA Kits
• Collagen III alpha 1/COL3A1 Proteins
• Collagen III alpha 1/COL3A1 Proteins and Enzyme