Collagen I alpha 1 Antibodies
Collagen type I (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is a fibril-forming collagen found in cartilage and connective tissues, expressed in fibroblasts, osteoblasts, and odontoblasts. We offer antibodies to detect Collagen I alpha 1 that have been cited in 65+ publications with 5+ customer reviews. Browse our extensive catalog for polyclonal antibodies, monoclonal antibodies and Collagen type I conjugated antibodies. Validated research applications include ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IHC-Frozen, Simple Western, and Western Blot. Our collagen alpha antibodies can be used in a variety of model species including Human, Mouse, Rat, Primate, Bovine, Equine, Canine, Avian, Feline, Rabbit, Porcine, and Sheep. Choose the anti-Collagen type I antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each Collagen I alpha 1 antibody is fully covered by our Guarantee+.
Alternate Names for Collagen I alpha 1 Antibodies
anti-Collagen I alpha 1 antibody, anti-COL1A1 antibody, anti-Alpha-1 type I collagen antibody, anti-Collagen 1 alpha 1 antibody, anti-Collagen 1 antibody, anti-collagen alpha 1 chain type I antibody, anti-collagen alpha-1(I) chain antibody, anti-collagen of skin, tendon and bone, alpha-1 chain antibody, anti-collagen, type I, alpha 1 antibody, anti-Collagen-1 antibody, anti-Collagen1 antibody, anti-OI4 antibody, anti-pro-alpha-1 collagen type 1 antibody
20 Results for "collagen-i-alpha-1" in Primary Antibodies
![Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Western-Blot-NBP1-30054-img0005.jpg "Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.")
![Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Immunohistochemical staining targeting Collagen I alpha 1 revealed severe disorganization of the annulus fibrosus (AF), and a decrease in staining intensity for group D. On the contrary, the arcshaped and layered AF structure with immunoreactivity for type I collagen staining were mostly maintained in group S when compared to group C. Presented images show the AF at site of surgery, defect, and transplant. Abbreviations: D: AF defect only group, S: AF defect treated with sheet transplantation group, C: sham group, NC: negative control of sample stained without primary antibody (scale bar=300um). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31463464/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30054-img0006.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30054-img0004.jpg)
![Simple Western: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Simple-Western-NBP1-30054-img0003.jpg)
![Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - TNC deficiency reduced kidney fibrosis in animal models.Deletion of TNC (TNC−/−) significantly attenuated the induction of collagen I assessed by IF following UUO by approximate 30% at day 7 & 10 compared with their wild-type littermates (A, n = 4 for each time point, two-way ANOVA p < 0.05). Consistently, the expression of fibrosis markers, such as collagen I alpha , fibronectin & plasminogen activator inhibitor-1 (PAI-1), were significantly lower in TNC−/− mice at UUO day 7 & 10 (B, n = 4 for each time point, two-way ANOVA, p < 0.05). Western blot showed that the proteins of collagen I alpha & alpha -SMA were also significantly reduced in TNC−/− mice at UUO day 7 (C, n = 7, p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36522320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30054_rabbit-polyclonal-collagen-i-alpha-1-antibody-31020241534311.jpg)
![Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-Frozen-NB600-450-img0003.jpg "Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000")
![Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of an FFPE ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was performed. Sample was incubated for 1 hour with primary antibody at a dilution of 1:400 at 20C.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-NB600-450-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for collagen I or for collagen III (NB600-594, RRID:AB_10001330) and imaged using fluorescent microscopy. Representative images (100 x 100 um) of each mouse strain and treatment group are provided for collagen I and collagen III immunofluorescence. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/abs/10.14814/phy2.14377) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunocytochemistry-Immunofluorescence-NB600-450-img0014.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-NB600-450-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-Collagen I alpha 1 antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunocytochemistry-Immunofluorescence-NB600-450-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunocytochemistry-Immunofluorescence-NB600-450-img0012.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and used at a dilution of 1:500.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-NB600-450-img0008.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Immunohistochemistry for type I collagen in keloid (20x). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28638180/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-NB600-450-img0013.jpg)
![Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Mouse healthy liver. Anibody at 1:200. Enzymatic/proteolytic antigen retrieval. No permeabilization. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-COL-1-Immunohistochemistry-Paraffin-NB600-450-img0015.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - FSP‐1–positive cells only partially responsible for collagen I & III production in irradiated bladders. Bladder strips from irradiated mice were costained for collagen I or for collagen III mRNA (pink) & for fibroblasts (FSP‐1, brown). U: urothelium; LP: lamina propria. Black arrow: FSP‐1 positive cells. Gray arrow: collagen only positive cells. Scale bar = 50 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-450_mouse-monoclonal-collagen-i-alpha-1-antibody-col-1-310202415175218.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-77457] - Collagen I Antibody [NBP1-77457] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Collagen-I-Antibody-Immunohistochemistry-NBP1-77457-img0002.jpg "Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-77457] - Collagen I Antibody [NBP1-77457] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NBP1-77457] - Collagen 1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77457-img0004.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-77458] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Collagen-I-Antibody-Immunohistochemistry-NBP1-77458-img0003.jpg "Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-77458] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NBP1-77458] - Collagen 1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77458-img0004.jpg)
![Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92858] - Western blot analysis of various lysates using Collagen I alpha 1 Rabbit pAb at 1:3000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): HT-29Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-92858_rabbit-polyclonal-collagen-i-alpha-1-antibody-4220251427520.jpg%3Fis_print=1 "Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92858] - Western blot analysis of various lysates using Collagen I alpha 1 Rabbit pAb at 1:3000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): HT-29Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92858] - Immunofluorescence analysis of NIH/3T3 cells using Collagen I alpha 1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92858_rabbit-polyclonal-collagen-i-alpha-1-antibody-42202514251851.jpg)
![Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92858] - Immunofluorescence analysis of NIH/3T3 cells using Collagen I alpha 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92858_rabbit-polyclonal-collagen-i-alpha-1-antibody-42202514264479.jpg)
![Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92858] - Immunofluorescence analysis of NIH/3T3 cells using Collagen I alpha 1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92858_rabbit-polyclonal-collagen-i-alpha-1-antibody-4220251431817.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92858] - Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using Collagen I alpha 1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92858_rabbit-polyclonal-collagen-i-alpha-1-antibody-4220251429044.jpg%3Fis_print=1)
![Western Blot: Collagen I alpha 1 Antibody [NBP1-68942] - Titration: 0.2-1 ug/ml, Positive Control: Human brain. Theoretical molecular weight: 139 kDa.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Western-Blot-NBP1-68942-img0004.jpg "Western Blot: Collagen I alpha 1 Antibody [NBP1-68942] - Titration: 0.2-1 ug/ml, Positive Control: Human brain. Theoretical molecular weight: 139 kDa.")
![ELISA: Collagen I alpha 1 Antibody [NBP1-68942] - Collagen I levels in rat brain cell lysates. ELISA image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-ELISA-NBP1-68942-img0005.jpg)
![Western Blot: Collagen I alpha 1 Antibody [NBP2-92877] - Analysis of extracts of various cell lines, using Collagen I/COL1A1 antibody at 1:2680 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Western-Blot-NBP2-92877-img0003.jpg "Western Blot: Collagen I alpha 1 Antibody [NBP2-92877] - Analysis of extracts of various cell lines, using Collagen I/COL1A1 antibody at 1:2680 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP2-92877] - Human breast cancer using Collagen I/COL1A1 Rabbit pAb at dilution of 1:10000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-92877-img0005.jpg)
![Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP2-92877] - Human colon using Collagen I/COL1A1 Rabbit pAb at dilution of 1:10000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-92877-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP2-92877] - Immunofluorescence analysis of NIH/3T3 cells using Collagen I alpha 1 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92877_rabbit-polyclonal-collagen-i-alpha-1-antibody-422025143183.jpg)
![Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Western-Blot-NBP2-29651-img0005.jpg "Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.")
![Immunohistochemistry: Collagen I alpha 1 Antibody [NBP2-29651] - Collagen I Antibody [NBP2-29651] - fibrotic mouse lung tissue showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-NBP2-29651-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NBP1-82488] - Staining of human cell line U-2 OS shows localization to cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82488-img0005.jpg "Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NBP1-82488] - Staining of human cell line U-2 OS shows localization to cytosol & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP1-82488] - Staining of human colorectal cancer shows strong strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82488-img0010.jpg)
![Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP1-82488] - Staining of human cervix shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82488-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NBP1-82488] - Staining of human colon shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82488-img0009.jpg)
