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CMP kinase Antibody

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Western Blot: CMP kinase Antibody [NBP3-38287] - Western blot analysis of various lysates using CMP kinase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunocytochemistry/ Immunofluorescence: CMP kinase Antibody [NBP3-38287] - Immunofluorescence analysis of MCF7 cells using CMP kinase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 ...read more
Immunoprecipitation: CMP kinase Antibody [NBP3-38287] - Immunoprecipitation analysis of 300 ug extracts of HT-29 cells using 3 ug CMP kinase antibody. Western blot was performed from the immunoprecipitate using CMP ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CMP kinase Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 69-228 of human CMP kinase (NP_057392.1).

Sequence:
LLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CMPK1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunoprecipitation 0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
  • Western Blot 1:500 - 1:2000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CMP kinase Antibody

  • CMKEC 2.7.4.14
  • CMPKUMKUMP-CMP kinase
  • cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
  • Cytidine monophosphate kinase
  • Cytidylate kinaseUMP/CMP kinase
  • Deoxycytidylate kinase
  • EC 2.7.4
  • UMP/CMPK
  • UMP-CMPK
  • UMPKUCK
  • Uridine monophosphate kinase
  • Uridine monophosphate/cytidine monophosphate kinase

Background

Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CMPK1