![Western Blot: DCK Antibody (10412) [NBP2-43639] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (10412) diluted at 1:500.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Western-Blot-NBP2-43639-img0008.jpg "Western Blot: DCK Antibody (10412) [NBP2-43639] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (10412) diluted at 1:500.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | 10412 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DCK |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Masashi Okada |
WB | Human | 09/14/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DCK |
![Immunocytochemistry/Immunofluorescence: DCK Antibody (10412) [NBP2-43639] - DCK antibody (10412) detects DCK protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: DCK protein stained by DCK antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Immunocytochemistry-Immunofluorescence-NBP2-43639-img0007.jpg "Immunocytochemistry/Immunofluorescence: DCK Antibody (10412) [NBP2-43639] - DCK antibody (10412) detects DCK protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: DCK protein stained by DCK antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Human lung cancer. DCK antibody [10412] diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Immunohistochemistry-Paraffin-NBP2-43639-img0002.jpg "Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Human lung cancer. DCK antibody [10412] diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at a dilution of 1:500.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Western-Blot-NBP2-43639-img0003.jpg "Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at a dilution of 1:500.")
![Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [10412] diluted at a dilution of 1:500.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Western-Blot-NBP2-43639-img0004.jpg "Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [10412] diluted at a dilution of 1:500.")
![Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [10412] diluted at a dilution of 1:500.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Western-Blot-NBP2-43639-img0005.jpg "Western Blot: DCK Antibody (10412) [NBP2-43639] - Analysis of various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [10412] diluted at a dilution of 1:500.")
![Western Blot: DCK Antibody (10412) [NBP2-43639] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Western-Blot-NBP2-43639-img0006.jpg "Western Blot: DCK Antibody (10412) [NBP2-43639] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Human colon carcinoma. DCK antibody [10412] diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Immunohistochemistry-Paraffin-NBP2-43639-img0001.jpg "Immunohistochemistry-Paraffin: DCK Antibody (10412) [NBP2-43639] - Human colon carcinoma. DCK antibody [10412] diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunoprecipitation: DCK Antibody (10412) [NBP2-43639] - Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 ug of DCK antibody (10412) or DCK antibody (NBP2-16108). Western blot analysis was performed using DCK antibody (10412) diluted at 1:500.](http://images.novusbio.com/fullsize/DCK-Antibody-10412-Immunoprecipitation-NBP2-43639-img0009.jpg "Immunoprecipitation: DCK Antibody (10412) [NBP2-43639] - Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 ug of DCK antibody (10412) or DCK antibody (NBP2-16108). Western blot analysis was performed using DCK antibody (10412) diluted at 1:500.")
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