![Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.Lane 1: PGM1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PGM1-Antibody-3B8-H4-Western-Blot-H00005236-M01-img0013.jpg "Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.Lane 1: PGM1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP, KD |
| Clone | 3B8-H4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | PGM1 (AAH19920, 1 a.a. ~ 562 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVKIVTVKTQAYQDQKPGTSGLRKRVKVFQSSANYAENFIQSIISTVEPAQRQEATLVVGGDGRFYMKEAIQLIARIAAANGIGRLVIGQNGILSTPAVSCIIRKIKAIGGIILTASHNPGGPNGDFGIKFNISNGGPAPEAITDKIFQISKTIEEYAVCPDLKVDLGVLGKQQFDLENKFKPFTVEIVDSVEAYATMLRSIFDFSALKELLSGPNRLKIRIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKYGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT |
| Specificity | PGM1 - phosphoglucomutase 1 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PGM1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PGM1 Antibody (H00005236-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of monoclonal antibody to PGM1 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/PGM1-Antibody-3B8-H4-Immunocytochemistry-Immunofluorescence-H00005236-M01-img0008.jpg "Immunocytochemistry/Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of monoclonal antibody to PGM1 on HeLa cell. Antibody concentration 20 ug/ml.")
![Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi ( Cat # H00005236-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody (M01), clone 3B8-H4 (Cat # H00005236-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PGM1-Antibody-3B8-H4-Western-Blot-H00005236-M01-img0010.jpg "Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi ( Cat # H00005236-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody (M01), clone 3B8-H4 (Cat # H00005236-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - PGM1 monoclonal antibody (M01), clone 3B8-H4 Analysis of PGM1 expression in Jurkat.](http://images.novusbio.com/fullsize/PGM1-Antibody-3B8-H4-Western-Blot-H00005236-M01-img0012.jpg "Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - PGM1 monoclonal antibody (M01), clone 3B8-H4 Analysis of PGM1 expression in Jurkat.")
![Immunoprecipitation: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 transfected lysate using anti-PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PGM1-Antibody-3B8-H4-Immunoprecipitation-H00005236-M01-img0009.jpg "Immunoprecipitation: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 transfected lysate using anti-PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 MaxPab rabbit polyclonal antibody.")
![Sandwich ELISA: PGM1 Antibody (3B8-H4) [H00005236-M01] - Detection limit for recombinant GST tagged PGM1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PGM1-Antibody-3B8-H4-Sandwich-ELISA-H00005236-M01-img0011.jpg "Sandwich ELISA: PGM1 Antibody (3B8-H4) [H00005236-M01] - Detection limit for recombinant GST tagged PGM1 is 0.3 ng/ml as a capture antibody.")
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