![Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-25386_rabbit-cebp-alpha-mab-hl1747-azide-and-bsa-free-212024941449.jpg "Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
| Reactivity | Hu, Rt, Mu, Bv, ShSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | HL1747 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CEBP alpha. The exact sequence is proprietary. |
| Predicted Species | Mouse (93%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CEBPA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CEBP alpha Antibody (NBP3-25386)Find related products by research area.
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Transcription Factor Antibodies Used In Landmark Evolutionary Study We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CEBPA |
![Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25386_rabbit-cebp-alpha-mab-hl1747-azide-and-bsa-free-2120249362766.jpg "Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - C/EBP alpha antibody [HL1747] detects C/EBP alpha protein at nucleus by immunofluorescent analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: C/EBP alpha stained by C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25386_rabbit-cebp-alpha-mab-hl1747-azide-and-bsa-free-2120249371523.jpg "Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - C/EBP alpha antibody [HL1747] detects C/EBP alpha protein at nucleus by immunofluorescent analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: C/EBP alpha stained by C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:500. Blue: Fluoroshield with DAPI .")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")