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Cdc23 Antibody

Images

 
Immunohistochemistry: Cdc23 Antibody [NBP3-35501] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using Cdc23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M ...read more
Immunohistochemistry: Cdc23 Antibody [NBP3-35501] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using Cdc23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with ...read more
Immunohistochemistry: Cdc23 Antibody [NBP3-35501] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Cdc23 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M ...read more
Western Blot: Cdc23 Antibody [NBP3-35501] - Western blot analysis of various lysates using Cdc23 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Cdc23 Antibody Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Cdc23 (NP_004652.2).

Sequence:
HSSKWSAELAFSLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGPLEKGQVKNEA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC23
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • Western Blot 1:500 - 1:2000
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for Cdc23 Antibody

  • ANAPC8anaphase promoting complex subunit 8
  • Anaphase-promoting complex subunit 8
  • APC8CDC23 (cell division cycle 23, yeast, homolog)
  • cell division cycle 23 homolog (S. cerevisiae)
  • cell division cycle protein 23 homolog
  • CUT23
  • Cyclosome subunit 8

Background

CDC23/APC8 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC23