![Western Blot: TPR Antibody [NB100-2866] - Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TPR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TPR-Antibody-Western-Blot-NB100-2866-img0005.jpg "Western Blot: TPR Antibody [NB100-2866] - Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TPR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 2275 and 2325 of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175). |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TPR |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in ICC/IF reported in scientific literature (PMID 24344181) and customer review. TPR antibody validated for KD from a verified customer review. |
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| Theoretical MW | 267 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af009_human-neurophysin-ii-affinity-purified-polyclonal-ab-immunohistochemistry-2812202013195.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Human | 01/24/2018 |
Summary
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reviewed by:
Verified Customer |
IF | Human | 08/27/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 01/24/2018 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 08/27/2014 |
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| Application: | IF | |
| Species: | Human |
| Gene Symbol | TPR |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: TPR Antibody [NB100-2866] - TPR and Mad1 interaction at the Nuclear Pores (green dots). DNA counterstained with DAPI (blue). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TPR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2866-img0002.jpg "Immunocytochemistry/Immunofluorescence: TPR Antibody [NB100-2866] - TPR and Mad1 interaction at the Nuclear Pores (green dots). DNA counterstained with DAPI (blue). Image submitted by a verified customer review.")
![Western Blot: TPR Antibody [NB100-2866] - Detection of Human TPR on HeLa whole cell lysate using NB100-2866. TPR was also immunoprecipitated by rabbit anti-TPR antibodies NB100-2864, NB100-2865 and NB100-2867.](http://images.novusbio.com/fullsize/TPR-Antibody-Western-Blot-NB100-2866-img0003.jpg "Western Blot: TPR Antibody [NB100-2866] - Detection of Human TPR on HeLa whole cell lysate using NB100-2866. TPR was also immunoprecipitated by rabbit anti-TPR antibodies NB100-2864, NB100-2865 and NB100-2867.")
![Knockdown Validated: TPR Antibody [NB100-2866] - Lane 1 contians wildtype MDA-MB-231 cell lysate nuclear extract. Lanes 2, 3 and 4 contain the TPR knocked down MDA-MB-231 cell lysate nuclear extract. The lowered intensity of the bands in knocked down cell lines compared to wildtype supports antibody specificity to intended target protein. Image was submitted by customer review.](http://images.novusbio.com/fullsize/TPR-Antibody-Knockdown-Validated-NB100-2866-img0004.jpg "Knockdown Validated: TPR Antibody [NB100-2866] - Lane 1 contians wildtype MDA-MB-231 cell lysate nuclear extract. Lanes 2, 3 and 4 contain the TPR knocked down MDA-MB-231 cell lysate nuclear extract. The lowered intensity of the bands in knocked down cell lines compared to wildtype supports antibody specificity to intended target protein. Image was submitted by customer review.")
![Western Blot Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Western_Blot_10680.jpg)
![Immunohistochemistry Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Immunohistochemistry_10732.jpg)
![ELISA Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_ELISA_22233.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
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