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Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]

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Western blot shows lysates of human small intestine tissue and plasma. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for precursor Apolipoprotein C-II/ApoC2 at approximately 12 kDa and processed ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of human small intestine tissue and plasma. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Biotin
BAF4497
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Carrier Free
AF4497-SP
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Apolipoprotein C-II/ApoC2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ApoC2
Thr23-Glu101
Accession # P02655
Specificity
Detects human ApoC2 in Western blots. In Western blots, this antibody detects both the precursor and processed forms of ApoC2.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
APOC2
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publication using
AF4497 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]

  • APC2
  • APOC2
  • Apo-CII
  • apoC-II
  • Apolipoprotein C2
  • Apolipoprotein CII
  • Apolipoprotein C-II
  • MGC75082

Background

Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol APOC2
Uniprot