![Western Blot: Cdc23 Antibody [NB100-59829] - Detection of human and mouse CDC23/APC8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Cdc23-Antibody-Western-Blot-NB100-59829-img0003.jpg "Western Blot: Cdc23 Antibody [NB100-59829] - Detection of human and mouse CDC23/APC8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human cell division cycle 23 (anaphase-promoting complex subunit 8) using the numbering given in entry NP_004652.2 (GeneID 8697). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CDC23 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 69 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cdc23 Antibody (NB100-59829)Find related products by research area.
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![Western Blot: Cdc23 Antibody [NB100-59829] - Detection of Human and Mouse CDC23/APC8 on HeLa whole cell lysate using NB100-59829. CDC23/APC8 was also immunoprecipitated by rabbit anti-CDC23/APC8 antibody NB100-59830.](http://images.novusbio.com/fullsize/Cdc23-Antibody-Western-Blot-NB100-59829-img0002.jpg "Western Blot: Cdc23 Antibody [NB100-59829] - Detection of Human and Mouse CDC23/APC8 on HeLa whole cell lysate using NB100-59829. CDC23/APC8 was also immunoprecipitated by rabbit anti-CDC23/APC8 antibody NB100-59830.")
![Immunoprecipitation: Cdc23 Antibody [NB100-59829] - Detection of human CDC23/APC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for IP at 3 ug per reaction. CDC23/APC8 was also immunoprecipitated by another rabbit anti-CDC23/APC8 antibody. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Cdc23-Antibody-NB100-59829-img0004.jpg "Immunoprecipitation: Cdc23 Antibody [NB100-59829] - Detection of human CDC23/APC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for IP at 3 ug per reaction. CDC23/APC8 was also immunoprecipitated by another rabbit anti-CDC23/APC8 antibody. Detection: Chemiluminescence with an exposure time of 75 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")